Winogradsky column lab page!


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Welcome to the Winogradsky column lab page! Students from the Departments of Biological Applications and Technology, University of Ioannina and Icthyology and Aquatic Environment, University of Thessaly, Greece and the Microbiology course, Faculty of Sciences, University of Cádiz, Spain, discuss their findings on Winogradsky columns they constructed!

If you want to add a post, please feel free to contact the blog administrators (Hera Karayanni, Sokratis Papaspyrou or Kostas Kormas)!



Καλωσορίσατε στη σελίδα των Winobloggers! Διαδικτυακός τόπος συνάντησης φοιτητών, φοιτητριών και διδασκόντων δύο Τμημάτων από την Ελλάδα: Tμήμα Βιολογικών Εφαρμογών και Τεχνολογιών, Παν/μιο Ιωαννίνων και Τμήμα Γεωπονίας, Ιχθυολογίας και Υδάτινου Περιβάλλοντος, Παν/μιο Θεσσαλίας και ενός από την Ισπανία: Σχολή Θετικών Επιστημών, Πανεπιστήμιο του Cadiz. Παρακολουθούμε, σχολιάζουμε, ρωτάμε, απαντάμε σχετικά με τα πειράματά μας, τις στήλες Winogradsky!


Bienvenidos a la pagina web de los Winobloggers! Aquí los estudiantes y profesores de dos departamentos griegos, el Departamento de Aplicaciones y Tecnologías Biológicas de la Universidad de Ioannina y el Departmento de Agricultura, Ictiología y Sistemas Acuáticos de la Universidad de Thessalia, junto con los estudiantes de Microbiología de la Facultad de Ciencias en la Universidad de Cádiz, se reúnen para observar, comentar, preguntar y responder a preguntas relacionadas con nuestro experimento, la columna Winogradsky.


Winogradksy columns

Winogradksy columns
'In the field of observation, chance only favors the prepared mind' Pasteur 1854

Blog posts

Saturday 27 June 2015

Finally, after 5 months my column became colorful. I assume due to high temperature and more constant day light!


Top left: 15/1/2015
Top right and bottom: 27/6/2015

Thursday 11 June 2015



Winogradksy column
     Team 14 (Papaioannou Aristeidis- Papadopoulos Panagiotis)

Hello Winobloggers,
We are a two person team from university of Thessaly in Greece and we are doing an experiment with the Winogradsky column. The experiment took place in the area of Volos Anavros. We used:  one plastic bottle of 1.5l,  one plastic bottle of 0.5l, one egg and Some small paper pieces.  we left our columns in a light place and took some photos during the days of the experiment.

Day 1.
At the first day of our experiment we placed our column in a bright spot.


Day 4.
After 4 days we noticed some changes in the colour of the water. It became more yellow.




Day 47.
 The black color of the sediment  is caused by the presence of FeS from the anaerobic respiration.


Day 55
 We can see the black colour of the sediment in the whole bottle and the cloudy water because of the anaerobic conditions in the column.



Wednesday 10 June 2015

Hello friends,
Winogradsky column by N. Xaxiri and P. Papageorgiou 
Our experiment contains sea water, sand, piec
es of paper and egg. We keep it in a darkness place.
  Day 1.At the first day


 Afterward,the color change into dark until it will become totally black with strange smell.

Winogradsky column

Hello friends,
Winogradsky column by C. Theochari and M. Zouroufidis
  • Our samples were taken by Anauros (local beach of Volos, Greece) during our university field trip. We already had prepared the necessary tools:
  •    1One plastic bottle of 1.5l
2  One plastic bottle of 0.5l

3 One egg

4  Some small paper pieces

We went there and we followed specific steps: 
Gather sea sediment  and make a hole in the middle
Put the paper and the egg inside, make a mixture
Put it inside the bottle (2/3 parts of the big bottle)
Finally, filled up with water.




Then we left it in a dark  place and took some photos during the days of the experiment.

                        Day 1.At the first day of our experiment we placed our column in a dark spot and sealed

15-20 th day:We noticed that the 3/4 of sediment became darker from the rest.

After 1 and a half month: Finally We have noticed that all the column has become black due to the presence of Fes in the sediment.

Monday 8 June 2015

Fresh from the press

Hola a todos! Καλημέρα σε όλους!

I just found this paper!!!

Abbasian F, Lockington R, Mallavarapu M & Naidu R (2015) A pyrosequencing-based analysis of microbial diversity governed by ecological conditions in the Winogradsky column. World Journal of Microbiology and Biotechnology 31: 1115-1126.

ABSTRACT
The Winogradsky column is used as a microcosm to mimic both the microbial diversity and the ecological relationships between the organisms in lake sediments. In this study, a pyrosequencing approach was used to obtain a more complete list of the microbial organisms present in such columns and their ratios in different layers of this microcosm. Overall, 27 different phyla in these columns were detected in these columns, most (20 phyla) belonged to bacteria. Based on this study, Proteobacteria (mostly Sphingomonadales), Cyanobacteria (mostly Oscillatoriales) and Bacteroidetes (mostly Flavobacteriales) were the dominant microorganisms in the water, middle, and bottom layers of this column, respectively. Although the majority of organism in the water layer were photoautotrophic organisms, the ratio of the phototrophic organisms decreased in the lower layers, replaced by chemoheterotrophic bacteria. Furthermore, the proportion of aerobic chemoheterotrophic bacteria was greater in the higher layers of the column in comparison to the bottom. The green and purple sulfur phototrophic bacteria inhabited the bottom and middle of these columns, with none of them found in the water layer. Although the sulfur oxidizing bacteria were the dominant chemolithotrophic bacteria in the water layer, their ratio decreases in lower layers, being replaced with nitrogen oxidizing bacteria in the middle and bottom layers. Overall, the microbial population of these layers changes from a phototrophic and aerobic chemoheterotrophic organisms in the water layer to a mostly anaerobic chemoheterotrophic population of bacteria in the bottom layers.


http://link.springer.com/article/10.1007%2Fs11274-015-1861-y#

Saturday 6 June 2015

WINOGRARDSKY COLUMN (TEAM 18)

Team 18 Konstantina Danae Chalkia-Eirini Aggeliki Charoudi Hello Winobloggers, We are a two person team from university of Thessaly in Greece and we are doing an experiment with the Winogradsky column.The experiment took place in the area of Volos Anavros.We used 1 bottle(1,5 L) from which we removed the upper part, seawater (~500ml),1 egg ,pieces of newspaper and an amount of sediment from the beach of Anavros that covered the 1/4 of the bottle.


  • FIRST DAY(18/3/2015)









At the first day of the experiment we placed the sealed bottle near to the window in order to be exposed to the sunlight.


  • AFTER 2 WEEKS

At the bottom of the bottle started to apear small black spots due to the appearance of the FeS from the anaerobic processes which are taking place inside the column.The colour of the water hasn't changed.

  • AFTER 4 WEEKS






The black spots have spreaded at the sediment but there has not been noticed turbidity in the water.

Thursday 4 June 2015




Lab report in Microbial Ecology:
Group: Maria -xristina Zerma, Dimosthenis Zairis
 
In this column Winogradsky, used sediment and egg and water from beach Anavros in Volos.
 
Day 1 :

During the first day , the column was sealed and placed in a bright place , at room conditions

Second week:
 
During the second week , an increase in turbidity of the water. The turbidity due to increased phytoplankton cells. 
As shown in the photo below shows a pale green color.
 


 
Third week
 
During the third week , the turbidity of the water was reduced significantly and the water surface formed plates.
 


 
 
Fourth week
 


After the fourth week, the precipitate gradually column acquired along the length of black simultaneously,
 the water column is also colored black.