I'm Any Athanasopoulou from university of Ioannina in Greece. I received the mixture of water and mud from the bank of Voidomatis river (Voidomatis bridge) on 10.23.2015. My column also contains enrichment substrates, such as pieces of paper, 1 egg with its shell and 1/2 sachet of baking powder. First I made a mixture of the river mud, the enrichment substrates and water and put it in the bottle with a funnel (in order to cover 1/3 of the plastic bottle ). Then I added extra mud till 2/3 of the bottle to be filled and finally I filled up with water. Since my column was ready, I put it in the attic of my house, close to a western window, so that it's exposed to sunlight and room temperature (average growth temperature 20ο C).
So far most of the water column is colored bright red probably by a large population of purple non-sulphur bacteria. I'm expecting growth inhibition of sample's psychrothropic bacteria, since the growth temperature is significantly higher than the river's cold water. At the expense of these is expected the developing of different varieties of bacteria, that are favored by this temperature change.
here is my bottle at the 8th week of the experiment: