Winogradsky column lab page!

Welcome to the Winogradsky column lab page! Students from the Departments of Biological Applications and Technology, University of Ioannina and Icthyology and Aquatic Environment, University of Thessaly, Greece and the Microbiology course, Faculty of Sciences, University of Cádiz, Spain, discuss their findings on Winogradsky columns they constructed!

If you want to add a post, please feel free to contact the blog administrators (Hera Karayanni, Sokratis Papaspyrou or Kostas Kormas)!

Καλωσορίσατε στη σελίδα των Winobloggers! Διαδικτυακός τόπος συνάντησης φοιτητών, φοιτητριών και διδασκόντων δύο Τμημάτων από την Ελλάδα: Tμήμα Βιολογικών Εφαρμογών και Τεχνολογιών, Παν/μιο Ιωαννίνων και Τμήμα Γεωπονίας, Ιχθυολογίας και Υδάτινου Περιβάλλοντος, Παν/μιο Θεσσαλίας και ενός από την Ισπανία: Σχολή Θετικών Επιστημών, Πανεπιστήμιο του Cadiz. Παρακολουθούμε, σχολιάζουμε, ρωτάμε, απαντάμε σχετικά με τα πειράματά μας, τις στήλες Winogradsky!

Bienvenidos a la pagina web de los Winobloggers! Aquí los estudiantes y profesores de dos departamentos griegos, el Departamento de Aplicaciones y Tecnologías Biológicas de la Universidad de Ioannina y el Departmento de Agricultura, Ictiología y Sistemas Acuáticos de la Universidad de Thessalia, junto con los estudiantes de Microbiología de la Facultad de Ciencias en la Universidad de Cádiz, se reúnen para observar, comentar, preguntar y responder a preguntas relacionadas con nuestro experimento, la columna Winogradsky.

Winogradksy columns

Winogradksy columns
'In the field of observation, chance only favors the prepared mind' Pasteur 1854

Blog posts

Monday, 19 March 2018

UCA_3C, 4C_1: Day 1. Column’s preparation.

- 20 g of Rio San Pedro’s sediment.
- Destilled water/sea water (group 4) and sea water (group 3).
- 0,3 grammes of filter paper (both groups)
- 0,1 grammes of CaSO4 and 0,3 grammes of SO4- (group 3)
- 100 grammes of sand.
The objective of this experiment is to recognise the different microorganisms depending on their metabolism, nutrition, etc. For that, we choose the nutrients and environment of the column depending on the microorganisms that we want to benefit.
We add 20 grammes of Rio san pedro’s sediment in a plastic recipient. With the purpose of getting it viscous, we mix it with some water. Each group add their chosen products (paper, sulfate…), and we keep mixing and adding water until necessary. We achieve a thick mass that we can get into the tube with the help of a funnel. Then we hit it softly so the sediment will go down on the tube, and the trapped bubbles go away. We mark the tube to indicate the level of the preparation, so we know where the nutrients are.
We prepare another mix with 20 grammes of sediment, 100 grammes of sand and some water, and we put it into the tube. On the top of the column, we add some more water (2 cm).
We think that , in the column with only filter paper, are going to grow the chemoorganoheterotroph organisms, and, in the other hand, in the column with sulfate, are going to grow the chemolithoheterotroph organisms.

UCA_8B,7B_Day 8

Winnogradsky column - Day 8

Group B8(Laura Lucena Del Amo, Noelia Moares Fernández)
GroupB7(Pablo Lucas Ezequiel Garin Ortega, Alba Mejías Gallardo)

We are back one week later to discuss about the changes that we have observed in both columns, the column exposed to sunlight (B8) and the column kept in darkness in which we added glucose (B7).

In the column exposed to sunlight (B8), which had Rio San Pedro sediment + 0.3 g paper + 0.1 g CaSO4, we find these characteristics:

  • The column is darker in the bottom, with black sediments. This probably is caused by the FeS precipitate, produced by anaerobic microorganisms from Fe (provided by the Rio San Pedro sediment) and SO4 (from the CaSO4). 
  • The top of the tube is much lighter than the bottom, due to the proliferation of photoautotroph microorganisms. These organisms produce O2, which helps keeping a gradient of oxygen.
  • Because of this gradient of oxygen aerobic microorganisms will grow close to the top of the column and anaerobic microorganisms will grow in the bottom.
  • There are a few of little bubbles in the column, they may be bubbles of N2 (from the sediment) as well as bubbles of H2S (mostly in the bottom).
  • The main difference observed this week is that we can perceive two different sides of the tube in the middle:
  1. One side lighter ( with an orange hue), this is the side exposed to sunlight. The explanation of this phenomenon is based in the fact that there might have proliferated photoorganoheterotrophic bacteria in the medium.
  2. The other side is darker, the same that we observed last week. There might be chemoorganoheterotroph microorganisms.
While in the B7 tube, kept in darkness (absence of light) and in which we added Rio San Pedro sediment + 0.3 g paper + 0.1 g CaSO4 + glucose, we find these changes:

Friday, 16 March 2018


In the A2 group's winogradsky column we added agar, yeast extract and skimmed milk powder. All this apart from the basic components: mud, sand, celullose and CaCO4.
We put this column to light. We have to see the differences between this column and the A1 group's column which have different components and is put to light too.

About column A2 we expect the appereance of  microorganisms able to demean lactose.

Thursday, 15 March 2018

UCA_8B,7B_Day 7

Winogradsky column – Day 7

Group B8 (Laura Lucena Del Amo and Noelia Moares Fernández) 
Group B7(Pablo Lucas Ezequiel Garín Ortega and Alba Mejías Gallardo)

One week after the preparation of the Winogradsky column, both groups have observed significant changes in our columns.
The changes of the group B7 (column in darkness) are described with details below:
  • We observed a huge amount of black sediments spread all over tube. This is caused by the reaction of H2S with Fe2+ resulting in HFe that presents a black color. This reaction is produced by anaerobic microorganisms. The Fe2+ comes from the sediment of San Pedro river, and the H2S is caused by the fermentation of present sugar in the column.
  • Many small bubbles appear, but there is a big bubble of gas that has separated in two the column. We think that these bubbles are formed by CO2 and CH4 probably due to the fermentation of the chemo-heterotrophs microorganisms.
  • we think that in the zones with lighter colors, the microorganisms chemo-heterotrophs haven't proliferated.
After the observation of the tube, we have provoked the exit of the gas bubble to prevent the spillage.

Now, we will explain closely the changes observed in the tube exposed to sunlight of group B8:
  • In the bottom of the column, we can observe an accumulation of black sediments. This indicates the proliferation of microorganisms able to breathe SO4- and make the reactions commented previously in this zone.
  • Also, we see bubbles in the middle of the colums. This gas may be N2 or H2S. We think that a part from these bubbles are formed by the sugar fermentation of micoorganisms chemoheterotrophs.
  • In the top of the column, we see a fine laver of lighter color. It can be provoked by photoautrophic microorganisms (anaerobic). They provide oxygen to the rest of the tube, creating a gradient of oxygen. Due to this fact, we will find aerobic organisms close to the top and anaerobic microoorganisms in the bottom.

Finally, we return the columns to their previous places and we'll wait for new changes in next week.

Tuesday, 13 March 2018

Winogadsky column. Day 7. UCA B1 B2

Hello everyone, we´re back a week later to check the progress of  our columns.

As we can see in the image, there are significant differences between both columns:
    - The column that had the teaspoon of salt didsfavoured the growth of microorganisms, maybe because of the osmosis.
    - In the column that hadn´t the salt we could appreciate a larger dark area at the bottom which is caused by microorganisms so we could say that they grew better in this column.

As we mentioned in the last blog  entry, both of our columns were exposed to sunlight so that it wasn´t a variable. It was done in this way so we could see how the salt affects the growth of microorganisms. 

Winogradsky column. Day 0. UCA B1 B2

Hello, we are groups B1 and B2 and we are going to explain how we made a Winogradsky column experiment.

       First of all, we added 20g of mud in our test tube. Later we mixed the mud with a little bit of San Pedro´s river water trying to get a viscous texture. The next step was adding 0,3g of paper and 0,1g of CaCO3. We prepared a 100g of sand and 20g of mud mix and we cleaned the test tube edges. In this moment wee got a 2 cm layer of water.

Hypothesis: The aim of this experiment is checking how the concentration of salt affects to microorganisms. For this, one group prepared a Wingardsky column without salt and the other group added a teaspoon of salt. Both columns were exposed to the sunlight.

Sunday, 11 March 2018

Winogradsky column. Day 0. UCA C2 C1

Helo! We are Rafael Pardo Velasco, Jaime Pérez Leiva, Blanca Ruiz Alonso, María Oliva Pareja González, Covadonga Muñoz Raya and Lorena Rodríguez Rivero! We will explain how we have made our Winogradsky column!

We have made our Winogradsky colum with:

Rio San Pedro sediment, 0,5g of CaCO3 and filter paper.

That colum has been made for this way:

- We put 20g of sediment and much water in a plastic container. When all the compounds became viscous, we put it in a test tube, covering ⅓ of it.
- We put slowly 0,5g of CaCO3 and much filter paper inside the tube. Before we had mixed them without introducing air bubbles.
- We carefuly hit the tube to send the sediment to the botton of the tube and to catch all the air bubbles.
-We washed the plastic container.
- We mixed 100g of sand and 20g of sediment without enriching.
- We repeated the third step and cleaned the tube borders.
- We waited for until all the compounds proned.
- A water layer was formed on the top of the tube.


Because of the addition of the filter paper and the CaCO3, we have created an environment that is enriched in CO2, furthermore, mixing all the compounds of our column, have ensured a fast diffusion of nutrients and gases inside the tube, so gradients are more widespread.

We made two Winogradsky columns for the same way to study the influence of light on them, putting one of our columns in light and the other in darkness. We supose that, in the column that we have put in darkness, it will inhibit the growth of photoautotrophic microorganisms. So, that column will only depend on quimioautotrophic microoorganisms, that pick up its energy of carbonated and sulfurated compounds. The other column will depend on light too.

Saturday, 10 March 2018



Hello lovers of microbiology

We are going to explain how we made our Winogradsky column experiment. 

First of all, we mixed in a bucket river “San Pedro”’s sediments and river water.  Then, we added 0,5g of NaCO3 and a piece of paper (cellulose). Finally, we mixed river’s sand we the previous mix (if it is necesary we add a little bit o water, but trying to not make de mix a liquid).

Then, we introduced the mix in a big test tube, in our case, we added 0,25g of NaCl for encourage the development of halophilous bacteriums.  For making it funnier we did two test tubes with the same composition, but one of them will be in the oscurity and we put the other in the light so we expect that in the second tube we will find more fotosintetic bacteriums so we can apreciate the differences between both tests tubes. 
What will happen the next time we observe them?

On the right we can see first a photo of the column that goes on the dark in day 0 and above we can see a photo of the column that goes on the light in day 0. 
On the right, we see the same columns but after 7 days. 

Friday, 9 March 2018

UCA_5A-6A-7A_1: Day 0 – Starting Winogradsky Column’s project!

Winogradsky Column Materials:

All groups included 40g Rio San Pedro sediment, 100g sand and sea water.

5A group’s column: 0,31g tryptone; 0,62g powdered milk; 0,1g CaSO4.

6A group’s column: 0,74g agar, 0,74g CaCO3.

7A group’s column: 0,1g CaSO4, 0,3g paper (cellulose).


1º-. We took off the solids residues from the sediment. Then, we mixed 20g of Rio San Pedro sediment with sea water, and after that we included the previous materials mentioned for each group.

2º-. Once the mixture was homogenized, we put it into a test tube. Secondly, we mixed 100g of sand and 20g Rio San Pedro sediment, with sea water. Both mixtures’ texture should be like milkshake’s texture. 

3º-. After that, we added the second mixture to test tube until we filled up to 2/3 of the test tube. We left the mixture stand a little bit. 

4º-. Finally, we added 2-3 cm of sea water on the top. 


5A: When the project has finished, we won't find aerobic bacterias owing to the plenty sources of N and S. In our column, two predominant colors will be found. In the lowest part of the test tube, there will be a black color which is caused by bacterias with a more anaerobic metabolism (respiration S2O3- -> H2S). It will take up about 1/3 of the solid part of the column. Above it, we will find a grey color, which is caused by other anaerobic bacterias (respiration NO3- -> NO2- -> N2).

6A: In conclusion, after having thrown to the column CaCO3 (calcium carbonate) and Agar the sample will keep the color it has because it does not have sulfur and although this in the dark it will not turn blue which would be the color it must acquire when the bacteria feed on sulfur.

7A: We thought that our column will have aerobic and anaerobic bacterias, due to the two feeding sources, the cellulose from the paper and the CaSO4. We also thought that our column will change its color because we will have anaerobic bacterias producing H2S from SO4-. Then the H2S will react with the iron in the mud to produce a dark-blue color. Besides, our column is exposed to sunlight. 

We will report more about our columns in the next entry coming soon.

 A5 Winogradksy Column.
 A6 Winogradsky Column
 A7 Winogradsky Column


San Pedro's River sediment + 0.3 g paper + 0.1 g CaSO4

Group 3B: Marcelo Gómez Herrera, Alvaro Lucero Garófano and José Luis Hernández Fernández.
Group 4B: Paula Gilabert Prieto and Pilar Grosso Rodríguez.

Hello everyone!

Today we will explane you how we have done ours Winogradsky's columns.

Firstly, we added San Pedro's river mud (1/3 of the length of the column), exactly 20 grammes.
We must remove big leaves and sticks.
Then we dilute the mud with a little bit of water from the river.
We didn't used distilled water due to the fact that the difference of concentrations will lead to lysis.
We added 0.1 grammes of CaSO4 and 0,3 grammes of paper(celulose). This is for create an atmosphere rich in sulphire and CO2.

Secondly we took another 20 mud grammes and we mixed it with sand.

Finally filled the column with mud until reaching to the top.


We have prepared 2 columns exactly with the same compositions. (Explaned in a few lines before)
The unique variable that can change the column is the light.
So we will keep one of them in contact with sunlight, and the other one will be kept in the darkness.
Because of that, the column kept in the light will grow photosyntetic organism, meanwhile in the darknesses will grow chemosyntetic organisms.