Welcome to the Winobloggers webpage! Students and professors from different universities meet to discuss their findings on Winogradsky columns they constructed!
We learn how to formulate hypotheses, observe, comment, ask and reply to questions related to our scientific experiments.
Καλωσορίσατε στη σελίδα των Winobloggers! Διαδικτυακός τόπος συνάντησης φοιτητών, φοιτητριών και διδασκόντων δύο Τμημάτων από την Ελλάδα: Tμήμα Βιολογικών Εφαρμογών και Τεχνολογιών, Παν/μιο Ιωαννίνων και Τμήμα Γεωπονίας, Ιχθυολογίας και Υδάτινου Περιβάλλοντος, Παν/μιο Θεσσαλίας και ενός από την Ισπανία: Σχολή Θετικών Επιστημών, Πανεπιστήμιο του Cadiz. Παρακολουθούμε, σχολιάζουμε, ρωτάμε, απαντάμε σχετικά με τα πειράματά μας, τις στήλες Winogradsky!
Bienvenidos a la pagina web de los Winobloggers! Aquí los estudiantes y profesores de dos departamentos griegos, el Departamento de Aplicaciones y Tecnologías Biológicas de la Universidad de Ioannina y el Departmento de Agricultura,Ictiologíay Sistemas Acuáticos de la Universidad de Thessalia, junto con los estudiantes de Microbiología de la Facultad de Ciencias en la Universidad de Cádiz, se reúnen para observar, comentar, preguntar y responder a preguntas relacionadas con nuestro experimento, la columna Winogradsky.
'In the field of observation, chance only favors the prepared mind' Pasteur 1854
UNIVERSITY OF IOANNINA GREECE BIOLOGICAL APLICATIONS AND TECHNOLOGY
Team 7: Serasidis Konstantinos, Tsinoglou Makrina
The sampling took place in Vrellis Lake of Ioannina on 21st of October 2016 at
around 1 o’clock.
For the composition of
the columns we used: 2 plastic
bottles (1,5 L) that the top was cut and removed, 2 raw
eggs, sheets of newspaper, a shovel, plastic bawl and plastic membrane.
After mixing the mud with the enrichment materials (egg and sheets of
newspaper), we filled 1/3 of the bottle with the mix, then we added mud without
enrichment materials for another 1/3 of the bottle. Finally, water from the
lake was added to fill the bottle. This method was used for the second column
In order to observe the development of the microorganisms without light
effect, one of the bottles was covered with foil. Both of the bottles were
placed at an outdoors environment.
In our experiment we will observe
possible differences at the growth of microorganisms in the two Winogradsky
columns, with presence of light at the first column and completely dark
environment at the second one. The hypothesis of the experiment is that algae and other aerobic
phototrophs will be present along the surface and water of the upper half of
the column that was held in light and absent in the column that was held in
complete dark. Green growth is often attributed to these organisms so the result
will be visually observed.
1. control column
2. column (dark)
After one week of development there were no noticeable
University of Ioannina Team A (Gregoris Notarides & Kostas Karanicolas)
In our experiment we will observe possible differences at the growth of microorganisms in two Winogradsky columns, presence of light at the first column and completely dark environment for the second one.
Sampling of mud and water done on 21th of October 2016 at 13:00 o'clock, in Vrellis Lake of Ioannina. For the creation of our Winogradsky's columns we used two plastic bottles of 1,5 L capacitive, where we put mud mixed with raw eggs and sheets of newspaper in small pieces.
We made three layers in our columns. First layer was the mud with the enrichment materials and second layer, simple mud without them until the 2/3 of the bottles. Third and final layer was water direct from the lake, without any subtraction of phytoplankton.
Our columns were placed in the laboratory, one in front of a window and the other inside a closet where the sun can't see it.
We expect the development of microorganisms in the column that is in front of the window will be faster and will give higher numbers, as we suppose that several species of microorganisms won't grow up in the second column where they will cannot perform photosynthesis without the presence of sun's light.
STUDENTS:NENA KOUKOUGELI DIMITRA-IOLI SKOUROLIAKOU UNIVERSITY OF IOANNINA GREECE BIOLOGICAL APLICATIONS AND TECHNOLOGY AQUATIC MICROORGANIMS Our experiment took place on 21/10/16 in
Vrellis lake of Ioannina at about 1.00 p.m. For our sampling, we used two
plastic bottles with capacity 1.5L each, which we filled up with mud and water
from the lake. For both bottles we followed the same procedure.
Except the 2 plastic bottles, we used 2
shovels, a bowl and of course… our hands, so we could collect the proper amount
of mud we needed. In order to create an enriched sludge we used 2 raw eggs (with
the yolks) as a source of Sulfur, 4 sheets of newspaper as a source of Carbon, and
grass which was consisted in the mud. The mixing happened with our bare hands
(it felt like we were 4 again). This enriched sludge, which was used as a
substrate, consisted the 2/8 of each bottle. Also, the 5/8 consisted of
non-enriched sludge and the rest 1/8 consisted of the lake water (approximately
First of all, we added the enriched sludge
and then we filled it up with the plane mud. After a while, we added the lake
water. Finally, we covered the top of the bottle with cellophane, in order to
achieve the long-term anoxic conditions.
As a result, we create two Winogradsky
columns, which we expose in two different conditions. The one, is near a window
in the Lab and the other one is near to a convertor (40oC). It is
important to point out that the second column is near to the conservator for 12
hours and the rest of the day is in standard conditions.
These pictures refer to the first day of the
1 Control column
2 warm-exposed column
The rapid fluctuation of the temperature on daily basis, reduces the growth
rate of the microorganism in the 2nd Winogradsky column.
experiment took place in Vrellis Lake of Ioannina on 21.10.2016 at 13:00
two plastic bottles (1,5L), a funnel, sediment from the lake (mud), a sheet of
newspaper, two whole eggs (with the pod) and water from the lake. After mixing
the sediment with the enrichment materials, we filled the ¾ of the first bottle
and then we added water from the lake. In the second one we filled the ¼ of the
bottle with the sediment and the enrichment materials and the other 2/4 without
them (just the sediment from the lake). After that we added water from the lake
till the top of the bottle. We left the bottle in a calm situation to
precipitateour sample and then we placed it in a spot with light
during the entire day and stable temperature.
expecting that microorganisms in the first bottle will grow up faster than the
second one because they have access in more nutritional ingredients (we provide them more carbon(newspaper) and sulphur(eggs)
source), or otherwise we will notice that so much quantity of nutritional
ingredients is a negative growth regulator.
Left one: bottle with ¾ sediment-enrichment materials and lake water
Right one: bottle with ¼ sediment-enrichment materials, 2/4 sediment and lake water
UNIVERSITY OF IOANNINA Sampling took place on 21.10.2016 at 13:00 in the Vrellis lake, in the region of Ioannina.The materials we used for the experiment were: 2 plastic bottles capacity 1.5L each, 1 shovel, a bowl (creating enriched sludge), a glass, a funnel, sludge from the lake, lake water, tap water, 2 egg yolks as a sulfur source, half a newspaper page as the cellulose source (coal). HYPOTHESIS: In the column in which was added water from the lake we expect several types of microorganisms to grow. Instead, the column in which was added tap water we are waiting not to observe growth of microorganisms. We are not yet able to make comments on the development of the column because the followin picture referes to the 1st day of the experiment .
LEFT BOTTLE: Lake water
RIGHT BOTTLE: Tap water