STUDENTS:NENA KOUKOUGELI
DIMITRA-IOLI SKOUROLIAKOU
UNIVERSITY OF IOANNINA GREECE
BIOLOGICAL APLICATIONS AND TECHNOLOGY
AQUATIC MICROORGANIMS
Our experiment took place on 21/10/16 in Vrellis lake of Ioannina at about 1.00 p.m. For our sampling, we used two plastic bottles with capacity 1.5L each, which we filled up with mud and water from the lake. For both bottles we followed the same procedure.
DIMITRA-IOLI SKOUROLIAKOU
UNIVERSITY OF IOANNINA GREECE
BIOLOGICAL APLICATIONS AND TECHNOLOGY
AQUATIC MICROORGANIMS
Our experiment took place on 21/10/16 in Vrellis lake of Ioannina at about 1.00 p.m. For our sampling, we used two plastic bottles with capacity 1.5L each, which we filled up with mud and water from the lake. For both bottles we followed the same procedure.
Except the 2 plastic bottles, we used 2
shovels, a bowl and of course… our hands, so we could collect the proper amount
of mud we needed. In order to create an enriched sludge we used 2 raw eggs (with
the yolks) as a source of Sulfur, 4 sheets of newspaper as a source of Carbon, and
grass which was consisted in the mud. The mixing happened with our bare hands
(it felt like we were 4 again). This enriched sludge, which was used as a
substrate, consisted the 2/8 of each bottle. Also, the 5/8 consisted of
non-enriched sludge and the rest 1/8 consisted of the lake water (approximately
0,3L).
First of all, we added the enriched sludge
and then we filled it up with the plane mud. After a while, we added the lake
water. Finally, we covered the top of the bottle with cellophane, in order to
achieve the long-term anoxic conditions.
As a result, we create two Winogradsky
columns, which we expose in two different conditions. The one, is near a window
in the Lab and the other one is near to a convertor (40oC). It is
important to point out that the second column is near to the conservator for 12
hours and the rest of the day is in standard conditions.
These pictures refer to the first day of the
experiment.
1 Control column 
2 warm-exposed column
Hypothesis:
The rapid fluctuation of the temperature on daily basis, reduces the growth
rate of the microorganism in the 2nd Winogradsky column.
Near the convertor...you mean at your place? This is why I counted one less column?
ReplyDelete