Hello everyone,
our names are Alvanou Nikoleta and Vardaka Panagiota and we are students of the department of biological applications and technology in Ioannina. We created three Winogradsky columns(1.5L each) in order to observe the bacterial growth in different temperatures and light conditions. The samples have been taken from Voidomatis river. Firstly, in all three columns we added mud mixtured with shredded parer as carbon source, an egg with its shell, 1/2 tea spoon soda and 25g baking powder as sulfur source and these ingredients covered 1/3 of the each bottles . Then we filled other 1/3 of each bottle with extra mud and added water from the river leaving some free space on the top. After that one of the columns was placed in the fridge in -12oC, the other one was placed in the balcony so it can be exposed to the sunlight (0-10oC), and the last one was covered with a black bag so its development wont be affected by the light (17-19oC). After three months in the first column was observed no development, at the second one the observed development was less than the expected and at the third one the development was not important as we expected.
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| The First column (-12oC) |
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| The Second Column (light) |
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| The Third Column (dark) |
The sediment is turning green close to the bottom in the second column. Am I right? What does this indicate?
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