Winogradsky’s Column

UNIVERSITY OF IOANNINA GREECE
BIOLOGICAL APLICATIONS AND TECHNOLOGY

AQUATIC MICROORGANISMS 

Team 7: Serasidis Konstantinos, Tsinoglou Makrina

The sampling took place in Vrellis Lake of Ioannina  on 21^st of October 2016 at around  1 o’clock.

For the composition of the columns we used: 2 plastic bottles (1,5 L) that the top was cut and removed, 2 raw eggs, sheets of newspaper, a shovel, plastic bawl and plastic membrane.

After mixing the mud with the enrichment materials (egg and sheets of newspaper), we filled 1/3 of the bottle with the mix, then we added mud without enrichment materials for another 1/3 of the bottle. Finally, water from the lake was added to fill the bottle. This method was used for the second column too.

In order to observe the development of the microorganisms without light effect, one of the bottles was covered with foil. Both of the bottles were placed at an outdoors environment. 

In our experiment we will observe possible differences at the growth of microorganisms in the two Winogradsky columns, with presence of light at the first column and completely dark environment at the second one. The hypothesis of the experiment is that algae and other aerobic phototrophs will be present along the surface and water of the upper half of the column that was held in light and absent in the column that was held in complete dark. Green growth is often attributed to these organisms so the result will be visually observed. 

1. control column

2. column (dark)

After one week of development there were no noticeable

differences between the two columns. 

1. control column

2. column (dark)

test

Winogradsky Experiment - TEAM A

University of Ioannina
Team A (Gregoris Notarides & Kostas Karanicolas)

Hello everyone.

In our experiment we will observe possible differences at the growth of microorganisms in two Winogradsky columns, presence of light at the first column and completely dark environment for the second one.

Sampling of mud and water done on 21th of October 2016 at 13:00 o'clock, in Vrellis Lake of Ioannina. For the creation of our Winogradsky's columns we used two plastic bottles of 1,5 L capacitive, where we put mud mixed with raw eggs and sheets of newspaper in small pieces.

We made three layers in our columns. First layer was the mud with the enrichment materials and second layer, simple mud without them until the 2/3 of the bottles. Third and final layer was water direct from the lake, without any subtraction of phytoplankton.


Our columns were placed in the laboratory, one in front of a window and the other inside a closet where the sun can't see it.

We expect the development of microorganisms in the column that is in front of the window will be faster and will give higher numbers, as we suppose that several species of microorganisms won't grow up in the second column where they will cannot perform photosynthesis without the presence of sun's light.

  STUDENTS:NENA KOUKOUGELI
                        DIMITRA-IOLI SKOUROLIAKOU
UNIVERSITY OF IOANNINA GREECE
BIOLOGICAL APLICATIONS AND TECHNOLOGY
AQUATIC MICROORGANIMS
Our experiment took place on 21/10/16 in Vrellis lake of Ioannina at about 1.00 p.m. For our sampling, we used two plastic bottles with capacity 1.5L each, which we filled up with mud and water from the lake. For both bottles we followed the same procedure.

 Except the 2 plastic bottles, we used 2 shovels, a bowl and of course… our hands, so we could collect the proper amount of mud we needed. In order to create an enriched sludge we used 2 raw eggs (with the yolks) as a source of Sulfur, 4 sheets of newspaper as a source of Carbon, and grass which was consisted in the mud. The mixing happened with our bare hands (it felt like we were 4 again). This enriched sludge, which was used as a substrate, consisted the 2/8 of each bottle. Also, the 5/8 consisted of non-enriched sludge and the rest 1/8 consisted of the lake water (approximately 0,3L).

  First of all, we added the enriched sludge and then we filled it up with the plane mud. After a while, we added the lake water. Finally, we covered the top of the bottle with cellophane, in order to achieve the long-term anoxic conditions.

  As a result, we create two Winogradsky columns, which we expose in two different conditions. The one, is near a window in the Lab and the other one is near to a convertor (40^oC). It is important to point out that the second column is near to the conservator for 12 hours and the rest of the day is in standard conditions.

  These pictures refer to the first day of the experiment.

1 Control column         

2 warm-exposed column

 Hypothesis: The rapid fluctuation of the temperature on daily basis, reduces the growth rate of the microorganism in the 2^nd Winogradsky column.

ISAVELLA ZACHARIADI

MARIA MASMANIDOU

UNIVERSITY OF IOANNINA

Our experiment took place in Vrellis Lake of Ioannina on 21.10.2016 at 13:00 o’clock.

We used two plastic bottles (1,5L), a funnel, sediment from the lake (mud), a sheet of newspaper, two whole eggs (with the pod) and water from the lake. After mixing the sediment with the enrichment materials, we filled the ¾ of the first bottle and then we added water from the lake. In the second one we filled the ¼ of the bottle with the sediment and the enrichment materials and the other 2/4 without them (just the sediment from the lake). After that we added water from the lake till the top of the bottle. We left the bottle in a calm situation to precipitate our sample and then we placed it in a spot with light during the entire day and stable temperature.

We are expecting that microorganisms in the first bottle will grow up faster than the second one because they have access in more nutritional ingredients (we provide them more carbon(newspaper) and sulphur(eggs) source), or otherwise  we will notice that so much quantity of nutritional ingredients is a negative growth regulator.

 
Left one: bottle with ¾ sediment-enrichment materials and lake water
Right one: bottle with ¼ sediment-enrichment materials, 2/4 sediment and lake water
 

Good luck with your experiments Ioannina.

Just found this on the web. Taking it up to the next level!

https://twitter.com/JulesDeep/status/790936758749888512



Regards,
Sokratis

WINOGRADSKY COLUMN EXPERIMENT 1st day

PYROVOLAKI NIKI
SKIADARESIS ARGIRIS
UNIVERSITY OF IOANNINA
Sampling took place on 21.10.2016 at 13:00 in the Vrellis lake, in the region of Ioannina.The materials we used for the experiment were: 2 plastic bottles capacity 1.5L each, 1 shovel, a bowl (creating enriched sludge), a glass, a funnel, sludge from the lake, lake water, tap water, 2 egg yolks as a sulfur source, half a newspaper page as the cellulose source (coal).
HYPOTHESIS: In the column in which was added water from the lake we expect several types of microorganisms to grow. Instead, the column in which was added tap water we are waiting not to observe growth of microorganisms.
We are not yet able to make comments on the development of the column because the followin picture referes to the 1st day of the experiment .




LEFT BOTTLE: Lake water
RIGHT BOTTLE: Tap water

New Winogradsky class at the University of Ioannina

A student not on the list!