Four
Winogradsy columns were constructed. (pictures from the columns can be found here:( https://drive.google.com/drive/folders/1PRv055DWphKSB6fsl9J6ypOXioezNHev?usp=sharing )
Researchers : George Kazantzidis , Giota Kontogeorgiou, Dimitris Papanikos.
Department of Biological Applications and Technologies
Hypothesis: Using
different color filters for each winogradsky column we expect different microorganisms growth depending on the color allowed in each column.
Materials
and methods
Place of experiment
Mud was
selected from Amvrakikos Lagoon western Greece (39° 0'25.69"Β,
20°55'7.19"Α) at 20/10/2017.
The weather was sunny and the place from which we
gathered the mud was wet but not mumbled. The place is known for high eutrophic levels. Also Amvrakikos lagoon is polluted cause many rivers from Western
Greece end to it, carrying a lot of fertilizers from crops.
Materials
Mud and water were taken from the lagoon. In the mud
was added 2gr of cellulose (crushed paper) and calcium carbonate (one egg, whole).
The mud was mixed in order to homogenize.
In each column (plastic bottle 1,5L) was filled 2/3 with mud (1L) and 1/3 with
water(0,5L). one column was used as standard and the others for the Hypothesis.
Each from the 3 columns was wrapped with plastic color filter membrane. The
three membranes used were blue (450nm), red (680nm) and green (520nm). All four
columns were placed in sunny place at marine biology lab (University of ioannina).
Results
Our first hypothesis was that in the standard column
we will find all kinds of bacterial communities. By contrast, in the green
column we expected to find only purple sulfur and non-sulfur bacteria in the
middle of the column because they are the only ones that can use the green light for
energy production. In the red column we expected to find green photosynthetic
bacteria and not purple bacteria (because purple bacteria does not absorb red
light). In the blue column we expected to find all types of bacteria but in
less abundance than in the standard column (blue light is absorbed by all kinds of
photosynthetic bacteria). We do not expected changes in the non-photosynthetic
bacteria communities.
Two months after the construction of the column we do not
observe any difference in our columns. We assume that is too early to have
bacterial growth because the membrane filters reflect a large amount of light
outside of the column.
One interesting result is at the water bacterial
communities which are grown at all columns but with different microorganisms
(observed with eyes) as they form different shapes of communities.
Since you did not observe any bacterial growth neither in the control nor in the filter covered columns -in contrast to other students-, could we suggest that light is limited in the hood????? I think that as you say, your columns probably need more time/light.
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