UCA_4B_3B_day7

San Pedro's River sediment + 0.3 g paper + 0.1 g CaSO4

Group 3B: Marcelo Gómez Herrera, Alvaro Lucero Garófano and José Luis Hernández Fernández.

Group 4B: Paula Gilabert Prieto and Pilar Grosso Rodríguez.

Hello everyone!

Today we will explane you how we have done ours Winogradsky's columns.

Firstly, we added San Pedro's river mud (1/3 of the length of the column), exactly 20 grammes.

We must remove big leaves and sticks.

Then we dilute the mud with a little bit of water from the river.

We didn't used distilled water due to the fact that the difference of concentrations will lead to lysis.

We added 0.1 grammes of CaSO4 and 0,3 grammes of paper(celulose). This is for create an atmosphere rich in sulphire and CO2.

Secondly we took another 20 mud grammes and we mixed it with sand.

Finally filled the column with mud until reaching to the top.

Hypothesis:

We have prepared 2 columns exactly with the same compositions. (Explaned in a few lines before)

The unique variable that can change the column is the light.

So we will keep one of them in contact with sunlight, and the other one will be kept in the darkness.

Because of that, the column kept in the light will grow photosyntetic organism, meanwhile in the darknesses will grow chemosyntetic organisms.