Winogradsky column lab page!

Welcome to the Winogradsky column lab page! Students from the Departments of Biological Applications and Technology, University of Ioannina and Icthyology and Aquatic Environment, University of Thessaly, Greece and the Microbiology course, Faculty of Sciences, University of Cádiz, Spain, discuss their findings on Winogradsky columns they constructed!

If you want to add a post, please feel free to contact the blog administrators (Hera Karayanni, Sokratis Papaspyrou or Kostas Kormas)!

Καλωσορίσατε στη σελίδα των Winobloggers! Διαδικτυακός τόπος συνάντησης φοιτητών, φοιτητριών και διδασκόντων δύο Τμημάτων από την Ελλάδα: Tμήμα Βιολογικών Εφαρμογών και Τεχνολογιών, Παν/μιο Ιωαννίνων και Τμήμα Γεωπονίας, Ιχθυολογίας και Υδάτινου Περιβάλλοντος, Παν/μιο Θεσσαλίας και ενός από την Ισπανία: Σχολή Θετικών Επιστημών, Πανεπιστήμιο του Cadiz. Παρακολουθούμε, σχολιάζουμε, ρωτάμε, απαντάμε σχετικά με τα πειράματά μας, τις στήλες Winogradsky!

Bienvenidos a la pagina web de los Winobloggers! Aquí los estudiantes y profesores de dos departamentos griegos, el Departamento de Aplicaciones y Tecnologías Biológicas de la Universidad de Ioannina y el Departmento de Agricultura, Ictiología y Sistemas Acuáticos de la Universidad de Thessalia, junto con los estudiantes de Microbiología de la Facultad de Ciencias en la Universidad de Cádiz, se reúnen para observar, comentar, preguntar y responder a preguntas relacionadas con nuestro experimento, la columna Winogradsky.

Winogradksy columns

Winogradksy columns
'In the field of observation, chance only favors the prepared mind' Pasteur 1854

Blog posts

Saturday, 18 February 2017



Team 9: Katerina Mironaki
             Levidiotis Charalampos

Hello everyone!
       Inevitably our semester long project comes to an end. We tried to delay our entry in hopes of having clearer and more definitive results. So without further ado, these are some pictures of our columns about 17 weeks from the beginning of the experiment. 
       Thankfully the results followed our prior assumptions a discernable growth spurt of microbes in the second column much sooner than the first one as well a more intense coloration of the column. “        
       By comparing the two columns someone can easily observe the advanced growth state that characterizes the second one. From the intense coloration of the water to the early appearance of the colonies of purple sulfur bacteria the second column kept surprising us. Day after day, week after week new things started to make their appearance (new colonies of microbes formed speckles and strips, the sediment began enriching, the bottle swelling etc). Some of these new “findings” cowardly appeared in the first column but with a notable delay in time.
       In the end we can say that we are pleased with our results but we needed more time(a pity that semesters aren't longer...not really). Nevertheless we learned a lot of things and had quite some fun during these months.

 Everyone, thanks and good luck with your own projects!   

                                                          Column 1



                                                              Column 2

Saturday, 11 February 2017

Tzani Kalliopi
Mavridi Olga
University of Ioannina Greece
Biological Application & Technology

WEEK: 8th
Left: Winogradsky column with egg
Middle: Winogradsky column with garlic
Right: Winogradsky column with egg & garlic 

The hypothesis stated was confirmed; we observed differentiation between the three bottles in microorganisms’ development. Specifically, in the bottle with the egg as a sulfur source, we saw green sulfur bacteria characterized by a green/olive colored zone, and red-purple sulfur bacteria growing. We also saw the formation of biofilm. In the bottle with garlic as a sulfur source, we saw green sulfur bacteria and red-purple sulfur. However, we also observed the formation of FeS. Also, the water was easier to observe, since there was little to none biofilm, and we saw the growth of a plant. Finally, in the third bottle, which had both egg and garlic, we saw green sulfur bacteria, red-purple sulfur bacteria, and purple non-sulfur bacteria characterized by a red/ orange or rust colored zone. Again, in the water, there was no biofilm formation, and it had a high cyanobacterial growth.
Hello fellow winobloggers! We are Kalliopi Tzani & Olga Mavridi, under graduate students in the field of Biological Applications & Technology in the University of Ioannina. In terms of a class/ curriculum concerning aquatic microorganisms, we were assigned to make a Winogradsky column. For this purpose, we placed lake soil and lake water, from the Vrelis’s lake, into three bottles in a ratio 2:1. Each bottle contained lake soil and newspaper as source of cellulose. The bottles, however, contained different sources of Sulfur. In the first one, we added an egg, with its pod. The second one contained garlic & the third one contained both of these sources of sulfur (egg & garlic). Our bottles were closed, as to not exchange gasses like oxygen, with the environment, and they were placed in a sunny room. We observed them in a weekly base and we stated the following hypothesis: if there will be a differentiation between the 3 bottles concerning microorganisms’ development.
Vrelis's pond

Winogradsky column with egg & garlic (t=0)

Winogradsky column with garlic (t=0)

Winogradsky column with egg (t=0)