Winogradsky column lab page!

Welcome to the Winogradsky column lab page! Students from the Departments of Biological Applications and Technology, University of Ioannina and Icthyology and Aquatic Environment, University of Thessaly, Greece and the Microbiology course, Faculty of Sciences, University of Cádiz, Spain, discuss their findings on Winogradsky columns they constructed!

If you want to add a post, please feel free to contact the blog administrators (Hera Karayanni, Sokratis Papaspyrou or Kostas Kormas)!

Καλωσορίσατε στη σελίδα των Winobloggers! Διαδικτυακός τόπος συνάντησης φοιτητών, φοιτητριών και διδασκόντων δύο Τμημάτων από την Ελλάδα: Tμήμα Βιολογικών Εφαρμογών και Τεχνολογιών, Παν/μιο Ιωαννίνων και Τμήμα Γεωπονίας, Ιχθυολογίας και Υδάτινου Περιβάλλοντος, Παν/μιο Θεσσαλίας και ενός από την Ισπανία: Σχολή Θετικών Επιστημών, Πανεπιστήμιο του Cadiz. Παρακολουθούμε, σχολιάζουμε, ρωτάμε, απαντάμε σχετικά με τα πειράματά μας, τις στήλες Winogradsky!

Bienvenidos a la pagina web de los Winobloggers! Aquí los estudiantes y profesores de dos departamentos griegos, el Departamento de Aplicaciones y Tecnologías Biológicas de la Universidad de Ioannina y el Departmento de Agricultura, Ictiología y Sistemas Acuáticos de la Universidad de Thessalia, junto con los estudiantes de Microbiología de la Facultad de Ciencias en la Universidad de Cádiz, se reúnen para observar, comentar, preguntar y responder a preguntas relacionadas con nuestro experimento, la columna Winogradsky.

Winogradksy columns

Winogradksy columns
'In the field of observation, chance only favors the prepared mind' Pasteur 1854

Blog posts

Monday, 30 March 2015

Cadiz, 7C

Hello everybody:
We saw for a time the Winograndsky column don’t change for this we decide to change the bottle on the other side and put in the window where it gives more the sun, since before it was located on the floor where it only gives thelight of the room… And few days later, because the last week was doing a bad day with storms and rains, when the sun go out our Winograndsky column change, how we can be seen in the pictures, our bottle isn’t totally black save the sediment goes to the bottoms of the bottle, in the beginning it sees the turbid water with a small roots, we believe this is a product for the reaction of the products that we put in it with the light of the sun, they make this phenomenon.

Cadiz, 4A: Weeks 2&3, Winogradsky Column, Group A4

Hello again, everyone! This is our second post about our Winogradsky column, and it has been two weeks, thus we will update the state of the bottle.

Just as a reminder, this is what our column was composed of: 
-Sandy Beach sediment from Rio San Pedro's beach
-Motor Oil
-CaCO3 and FeSO

At first, the bottle was practically dead, with just a few sulphur-reducing bacteria. However, in just two weeks, the bottle seems to be full of life. Lots of new colonies and bacteria have grown in both sides of the column, most of them sulphur-reducing. We can tell that because of the orange colour of the halo around it.

On the illuminated side, a big orange colony has grown in the upper side of the bottle, so we can say it uses oxygen to grow; but in the back side, the non-illuminated one, an insane amount of bacteria has grown, with a strong grey colour; whereas the bacteria follow a black/dark pattern; all surrounded by the already mentioned orange halo. The fact that they grow on the non-illuminated side means that they don’t need light to survive, therefore they are not photosynthetic, and we can see that they grow equally despite the oxygen variation along the bottle, so it is possible that they are anaerobic, yet tolerant, not strict.

Here are some photos of the column, taken after a week and a half:


In the left one we can see the big colony of the illuminated side; on the right one we observe the huge amount of grey and black bacteria that has grown on the dark side.

That is all for now, we will keep you updated with the state of the bottle, so see you all next week!

Wednesday, 25 March 2015

Post format

Hi everyone,

In order to be able to follow your posts more easily, it would be great of you would add your group number at the beginning of the post title.

Cadiz A1: Day 14: The column has changed!

I also suggest you add in the first line the composition of your sediment. For example,

Mud (creek) + CaCO3 + cellulose
Mud (hypersaline lagoon) + Beach sand + FeSO4 + ...

And below add your post.

Good work so far to all of you. Thank you for the participation!


Tuesday, 24 March 2015

Cadiz, 4B: third week results

Hi winobloggers! I’m Paula and it is the second time I write here. I was taking about the Winograndsky Column and today I’m going to bring you the results of the third week.
We haven’t seen big changes in the bottle maybe due to the fact that we mixed the mixture with oil motor. Because of that our bottle isn’t so spectacular like the other ones.
Despite that we appreciated that the liquid inside the bottle is more liquid than before as the water has spread out.
Another thing we noticed is that it seems like the bottle had expelled vapours and they had been dried. That has left red signs in the bottle. (Like redish dry sediment).
On the other hand, the sediment continues black like the other days although there are a part in the top that seems clearer than the rest, maybe because it is starting to appear life in the sediment.

These are some pictures of the bottle during the third week.

Sunday, 22 March 2015

Hello everyone!

That`s the first time we write about Winogradsky colum. We are a couple of students who are on their first year in Biotechnology in UCA.

Our colum was made with Rio San Pedro´s sediment, oil, strach and carbonate. First of all, we took the sediment and we diluted it with water. After that we added strach, oli and carbonate. We mixed it with our hands. Finally, we put that mixure in a clean empty bottle and we added water of that river too.

We brough it to our house and set it in a sunny place ( a terrace). Few days after that we could see the appearance of little black points, what we believe that are some photosyntetic bacterias. We could see that the water added changed its color to orange and we think it is caused by the bacteria´s breaht.

Cadiz, 6A: Winogradsky column biotechnology group A6, 1st and 2nd week.

Hello, we are a group of students of biotechnology and we have done as a practical microbiology, one Winogradsky column.
The sediments of the Winogradsky column were collected from a lagoon close to the faculty of science.The date on which began the experiment was 04/03/2015. The material that has been used in the preparation of the column has been:
- NaCl
- Engine Oil
- Starch
- FeSO4
- CaCO3
- Pieces of paper

The mixture is first prepared by throwing a little of each component and stirring, then added the rest and is returned to mix. The clay leaving already ready to be introduced in the bottle. The volume of sediment is three quarters of the bottle. The water that was added was collected from the same lagoon as the sediment. The column is in a room near the window, giving you the light of the sun, but does not directly. Always with the same area to light and the other in the shade. We add many different components to see which of the mircroorganismos are more likely to develop. We hope to see sulfate-reducing, iron-oxidizing bacteria, besides oil eating bacteria and facilitate obtaining carbon CaCO3 and starch.

We can skip the first week because the most important thing that has happened in the death of the worms had.

In the second week, the part that is the sun has taken a green brown traces along the whole bottle. On the other hand the private part of the sun has taken a grayish tone.

                                             Here we can see the area that faces to sunlight

                                 Furthermore, this is the part of the column is not exposed to light.

The column is always in this position, the blue arrows that appear at the top it is to know that area should expose the light if you move home from another member of the group.

PS: Sorry I took so long to join this blog and upload the data on my column.

Saturday, 21 March 2015

Cadiz, 8A: Winogradsky, Group A8 Biotechnology UCA

Hello Winobloggers.

We are a couple of students who are on their first year in Biotechnology in UCA. I'm going to talk about my Winogradsky column's evolution for these weeks.

First of all, our Winogradsky column has the following composition:

Sand from Rio San Pedro's beach (Cadiz).

Water from a lagoon. Water takes up about 3 centimeters of the bottle.

Iron sulfate

Pieces of filter paper

The mixture was made by this form:

Firstly, we put the sand into the bottle. It takes up about 2/3 of the total bottle's volume

The bottle was recolected in Laboratory 8 of Science's faculty (Pto Real) on the date of: 4- March- 2015 . Now, the bottle is ubicated at my home in El Pto de Sta Maria. It has the following ubication: