Hello winobloggers,
We apologize for the delay. This is our first post about the Winogradsky column. We are other three students of biotechnology in the University of cádiz.
We made our Winogradsky column on tuesday, 3th March in our Microbiology laboratory. Our column contains a mixture of muddy sediment from San Pedro river and muddy sediment from a hypersaline pond. Moreover, we added as enrichments : CaCO3, FeSO4 and NaCl(salt).
First, we mixed the sediment from the river and from the pond in a bucket.We put more sediment from the river and we mixed with our hands. Then, we added the enrichment (one teaspoon of each compounds) and we kneaded again. After that, we introduced the mix in a bottle (around two thirds) and finally, we poured 2 cm of water from the same river.
Our column is situated in a desk next to a window, where receives the sunlight only on one side.
The column has changed much in this week. The first day it has a grey colouration, the water was turbid and it had two worms. However the tuesday, 10th march, the water was yellowish, the column was dark grey with a reddish colouration on the side where receives the sunlight and black spots have appeared.
We think that in our Winogradsky column are living or will appear: cyanobacterias in the water (the aerobic zone);purple and green sulfer bacterias in the middle; sulfate-reducing bacteria in the bottom because we added FeSO4 and these bacterias producing a nauseating smell and the black spots that we can see; and ,maybe, halophile bacterias because we added salt. In summary, we think that in the future our Winogradsky column will probably be similar to the theoretical image.
Here you can see the images of our column:
03/03/2015
07/03/2015
10/03/2015
We apologize for the delay. This is our first post about the Winogradsky column. We are other three students of biotechnology in the University of cádiz.
We made our Winogradsky column on tuesday, 3th March in our Microbiology laboratory. Our column contains a mixture of muddy sediment from San Pedro river and muddy sediment from a hypersaline pond. Moreover, we added as enrichments : CaCO3, FeSO4 and NaCl(salt).
First, we mixed the sediment from the river and from the pond in a bucket.We put more sediment from the river and we mixed with our hands. Then, we added the enrichment (one teaspoon of each compounds) and we kneaded again. After that, we introduced the mix in a bottle (around two thirds) and finally, we poured 2 cm of water from the same river.
Our column is situated in a desk next to a window, where receives the sunlight only on one side.
The column has changed much in this week. The first day it has a grey colouration, the water was turbid and it had two worms. However the tuesday, 10th march, the water was yellowish, the column was dark grey with a reddish colouration on the side where receives the sunlight and black spots have appeared.
We think that in our Winogradsky column are living or will appear: cyanobacterias in the water (the aerobic zone);purple and green sulfer bacterias in the middle; sulfate-reducing bacteria in the bottom because we added FeSO4 and these bacterias producing a nauseating smell and the black spots that we can see; and ,maybe, halophile bacterias because we added salt. In summary, we think that in the future our Winogradsky column will probably be similar to the theoretical image.
Here you can see the images of our column:
So in just a week, we have a black, a green and a red column! See if you can beat that Volos! :-)
ReplyDeleteWhere does the red colour come from?
You also added CaCO3. How would it affect the column?
Sokratis
It is not well appreciated in the photos , but comparing both sides of the bottle was obvious the reddish color of the illuminated side.
ReplyDeleteWe are not sure about what's the bacteria that causes coppery color. According to the information we have found, it may be red not dependent on sulfur bacteria (Rhodospirillum) or sulfur-dependent bacteria (Chromatium).
The CaCO3 is a pH regulating agent. This maintains the pH optimum for bacterial survival.