Winogradsky column lab page!

Welcome to the Winogradsky column lab page! Students from the Departments of Biological Applications and Technology, University of Ioannina and Icthyology and Aquatic Environment, University of Thessaly, Greece and the Microbiology course, Faculty of Sciences, University of Cádiz, Spain, discuss their findings on Winogradsky columns they constructed!

If you want to add a post, please feel free to contact the blog administrators (Hera Karayanni, Sokratis Papaspyrou or Kostas Kormas)!

Καλωσορίσατε στη σελίδα των Winobloggers! Διαδικτυακός τόπος συνάντησης φοιτητών, φοιτητριών και διδασκόντων δύο Τμημάτων από την Ελλάδα: Tμήμα Βιολογικών Εφαρμογών και Τεχνολογιών, Παν/μιο Ιωαννίνων και Τμήμα Γεωπονίας, Ιχθυολογίας και Υδάτινου Περιβάλλοντος, Παν/μιο Θεσσαλίας και ενός από την Ισπανία: Σχολή Θετικών Επιστημών, Πανεπιστήμιο του Cadiz. Παρακολουθούμε, σχολιάζουμε, ρωτάμε, απαντάμε σχετικά με τα πειράματά μας, τις στήλες Winogradsky!

Bienvenidos a la pagina web de los Winobloggers! Aquí los estudiantes y profesores de dos departamentos griegos, el Departamento de Aplicaciones y Tecnologías Biológicas de la Universidad de Ioannina y el Departmento de Agricultura, Ictiología y Sistemas Acuáticos de la Universidad de Thessalia, junto con los estudiantes de Microbiología de la Facultad de Ciencias en la Universidad de Cádiz, se reúnen para observar, comentar, preguntar y responder a preguntas relacionadas con nuestro experimento, la columna Winogradsky.

Winogradksy columns

Winogradksy columns
'In the field of observation, chance only favors the prepared mind' Pasteur 1854

Blog posts

Friday, 27 April 2018


Tube in darkness
We can see that there's air due to the fact that there was too much sustrat and microorganisms have produced CH4 .
In this column all is dark, it has anaerobic bacteriums.

Tube in light
We can see that in the surface, the tube has turned to light brown due to the presence of aerobic bacteriums.

When we compared both columns, we can see that only in the light column has appeared an extract light brown. Therefore, we deduce that the microorganisms that have grown there, are photosyntetic.

 Darkness                                                                                                           Light

Tuesday, 24 April 2018


San Pedro's River sediment + 0.3 g paper + 0.1 g CaSO4

Group 3B: Alvaro Lucero Garófano and José Luis Hernández Fernández.
Group 4B: Paula Gilabert Prieto and Pilar Grosso Rodríguez.

Did you miss us? No problem, we are back again to show you the evolution of  ours Winogradsky's columns after 44 days 

We can appreciate several changes in both columns.

The column 3B, which was in contact with the light has developed different changes.

Firsly, all bacterias which has grown in the column are photosyntetic bacterias due to the fact that they are in contact with light. Then we can classify this bacterias in two groups.
Aerobic bacterias and anaerobic bacterias.
The aerobics bacterias are on the top of the column because all the oxygen of the column are on the top. 
Also we must emphasize that there are a gradient of oxygen in the column, and the more it descends in the column, the less oxygen are in it. The bacterias which are at the bottom maybe can use the sulphate as the last one who accepts electrons. So this is the reason why has grown bacterias in a different colour at the bottom.

The surface which has been in contact with light is lighter than those surface which has not been in contact with it. 

The column 4B, which was in the darkness has developed some changes.

First of all, we can see that  there are a gradrient of oxygen in the column again, so at the bottom has grown anaerboic bacterias.
the difference with the other column is that in this one has grown chemosyntetic organisms, so the colour of this column is darker than the other one. 

Finally, Ours firts ideas of what could happen, are being succesful with our result




Monday, 23 April 2018

UCA_5A_6A_7A: Day 44 Winogradsky column´s evolution after a month

Winogradsky column´s evolution after a month:

We are back again, and now, we are going to show the changes in our columns:

5A Winogradsky Column:

The group 5A let his Winogradsky column in an illuminated environment, and after one month, we can observe that there is a colour variation at the top of the test tube because it has acquired a red tonality in the liquid part. The solid part doesn't have colouring. It is quite reasonable, since we put a lot of N and S sources into the tube, therefore, there are a lot of anaerobic bacterias, which are responsible of the colour variation. Since the amount of anaerobic organisms is incredibly big, there aren't any kind of  aerobic organisms in our test tube.

6A Winogradsky Column:

The group 6A let his Winogradsky column in a dark environment, and after approximately 30 days, the column is a little bit darker. But it continues with a little brown part at the bottom, we think it's because there aren't too many nutrients, or it will be because in that zone has grown another variety of bacteria. Throughout the column we can observe various black points and it could be accumulations of bacteria.

7A Winogradsky Column:

The group 7A let his Winogradsky column in an illuminated environment. We have observed changes after a month. The side of the column, in which the light does not reach, has a grey colour produced by anaerobic bacterias. Also, the column has a red colour in the middle. We think it may be produced by aerobic bacterias, which produce a substance with this colour. Secondly, we noticed more bubbles produced with the CH4 that bacterias expel out. Finally, we conclude with a general development of aerobic and anaerobic bacterias through the column along this month, in a way that we expected.

6A Winogradsky Column

7A Winogradsky Column

5A Winogradsky Column