UCA_1B_4: Day 74: Civil War: Dark vs Light

Our column still remains the same and we don´t expect any more changes. Our column was in the dark side.
  
However, we can appreciate so many differences between our bottle and the one in the light side, which was the column made by the group 11B.

 

As in our column, in here we can see H₂S at the bottom of the bottle, but in this case it has appeared  another microorganisms that grew depending on the light they were receiving.

In the side where the light strikes directly, microorganisms with differents color have developed.

The dark violet patches are sign of the presence of the sulfur bacteria. Next to them some iron oxidizing bacteria have grown. They are orange and we think they are the same that were on the top of the column which stayed in the dark side, even though we don´t know why they are there.

On the other hand, the side where the bottle wasn´t receiving direct light, has an intense green color, corresponding to the green sulfur bacteria.

On the top of the bottle we can see heterotrophic bacteria, with a green and brown color.

UCA_GroupA2_Day 63_Rio San Pedro sediment + 1,5 g FeSO4 + paper_(column exposed to sunlight)

It has been sixty three days  since we prepared our column.

Unfortunately, we can not appreciate many changes since the last day we checked it.

At the top of the column we can see that the number of algae has decreased, one of the reasons could be that the column was not totally exposed to the sunlight, so, if there is not sunlight, there is no photosynthesis and due to this, the algae can not grow.

In the middle,green regions have become bigger than the last day so this indicate us that the cyanobacterias have grown more. When there is not oxygen, the  SO4^2-  can be used as a last electron acceptor. The red regions have also increased the size.

At the bottom, we can see that there are not any changes, the black color is the same.

UCA_GEN_5B-8B_4: Day 5

Day 5: 19/05/2016

LIGHT: We observed variation in the color of the column with brown colors that have become more orange, green colors have intensified and are present in most areas. There is a loss of volume of water and dead cyanobacteria.

DARKNESS: It remains the same but we can observe a strip of orange color in the bottle in the water, which is more turbid.

UCA_GEN_5B_8B_4: Day 4

Day 4: 27/04/2016

LIGHT: It has more greenish shades, there is loss of bacteria in the surface layer that has become reddish and yellowish. Sulfur disappears and has CO2 bubbles, surface water is also transparent.

DARKNESS: It is grey more clear and surface water presents a layer of microorganisms from Brown.

DIFFERENCES: After eight weeks we observe that the bottle exposed to light presents more greenish coloration and a loss of black (iron sulfide). Finally, the surface water is clear and there is loss of microorganisms.

The dark bottle presents a gray color more clear and on the surface turbidity has been lost.

UCA_EN_6&3-A_3: Day 28

Río San Pedro sediment + 2g CaSO₄ + filter paper

It passed a week since the last observation.

Light exposed column: the changes we can appreciate are an increase of the seaweed population in the very first phase, thanks also for the presence of photosynthetic bacteria developed in the column. In the first part of soil that is exposed to the light we notice a very bright orange color, that's for the development of oxidative bacteria. Way down from the column we realize an increase of dark spots thanks to the precipitation of iron sulfide.

Dark column: in the column leaves in dark conditions we have not noticed important changes, but the increase of dark spots by the end of the column thanks to iron sulfide's precipitation.

UCA_EN_ 3&6-A_2: Day 15

Río San Pedro sediment + 2g CaSO₄ + filter paper

Hello, by the first time we can observed our bottles, and this is that we saw:

-Conditions of daylight:

In the top we saw some seaweed and there is not turbidity.

We apreciated two zones. One of them where the sunligth pass around the bottle, we saw the effects of the photosintesis that the bacterias show. In the other where the ligth didn 't pass we saw a change of orange color owed an oxidative bacteries. Under these zone we saw black points that are iron sulfide (III) precipitate.

In the low zone we didn't see alterations, only the iron sulfide (III).

-Changes at darkness:

In this bottle we can appreciate diferences, there are turbidity owed by the microorganisms that are behind this zone and the next one. It's corresponded by the oxidative bacteries. In the middle zone there are not significative changes, there are more iron sulfide (III) cuantity In the low zone the iron sulfide (III) deposits.

    

UCA_6B_2: Day 28 - The Iron Strikes Back

Rio San Pedro sediment + 1g CaSO4

Our column has not changed very much these weeks. We visited it a few days ago and see that nothing changed but the little dark stains (which are the reduced iron) that are bigger now and there are more of them. Last week we took some photos to see the changes:

 Our column after a few weeks.

We can see that our column is darker than ever, and there are more little orange sediments on the top of the bottle.

Our column has not changed very much but in the colum of the group 10B (the light bottle one) that day we spot some little intruders. There were as we can see in the photo below some snails looking around in the top of the bottle. 

We suppose that this is due to the fact that the sediment was taken from the San Pedro's river and the snails were inside of it. We could saidly afirm that maybe there was snails inside our column too, but the lack of organic matter and light did not make the life easy in there for them. Now they may rest in peace.

A comparation of the columns.

Now, if we compare our column with the group 10B (light column) one, we can see that their column does not present big black stains. Also, their column have a green colour in contrast to our. This is because of the presence of photosynthetics microorganisms, that need chlorophyll to capture light photons

UCA_EN_ 3 & 6-A_1: Day 0

Río San Pedro sediment + 2g CaSO₄ + filter paper

Hi, we are a group of students of Enology and we have made a Winogradsky's column for the subject of microbiology.

First we split in two group, three of us did this column under conditions with daylight and three in darkness.

Then we started to make a column with a bottle by:

- Little pieces of paper and sediments (we use beach's land). And we mix this.

- We add 2g of CaSO₄ and mix again.

Next we fill the mix in the bottle but only the fourth part and fill in with sediments again until to complete almost all bottle. This time this sediment without paper and  without CaSO₄.

For the end we add 1cm of beach's water and close the bottle but not complete, we did this for the microorganisms can to breath.

Both groups did this but, like said before, one bottle put in the window for the daylight and the other in darkness. This way we can observed how grow the microorganims in conditions luminous and conditions of darkness and study the contrast between them.

We believe that we have more growth in the bottle of conditions luminous that the bottle in darknes because the firts can do the photosynthesis and use sunlight like power source.

We hope to see many changes in our bottles and tell you.

University of Thessaly, Volos, Group Number 2, Dark, Incubation number 57

University of Thessaly, Volos, Group Number 2, Dark, Incubation number 57

Hello to everyone. We are presenting you our results from day 25 to day 57

DAY 25                                                                                        

  The odor became more noticeable due to the presence of hydrogen sulfide (H2S). In addition the lower layer of the sediment became darker due to the presence of organic matter in the Winogradsky column. The color of the water became more lighter than it was the days before.

DAY 50

A beige-brown color replaced the previous one.Also the white foam along with the bubbles remain in the surface.

DAY 57

We can observe that the water is black now.Futhermore there is a foam-bubble crust on the surface. This is due to the death of microorganisms.

University of Thessaly, Group number 15 (Micheli Sophia-Maria, Mladeni Sofia) Day 0-52 (column exposed to light)

We are Mladeni Sofia and Micheli Sofia and we will present you our results between first days to 52th day.  Our experiment started at 21th of March on Anauro’s beach. We took the sediment from the bottom of the sea and mixed it with  pieces of newspaper and a broken egg.  We filled the 2/3 of a plastic bottle with the mixed sediment and filled the rest with sea water. We covered the column and placed it behind a window. The first days the sediment had a light grey-brown color and the water was light yellow (because of the broken egg). As the days passed, the color of the sediment began to darken until it became black. The water became after a period of 20 days it brown and now it is black.

day 14

day 20

day 20

day 32

day 52

day 52