Winogradsky column lab page!

Welcome to the Winogradsky column lab page! Students from the Departments of Biological Applications and Technology, University of Ioannina and Icthyology and Aquatic Environment, University of Thessaly, Greece and the Microbiology course, Faculty of Sciences, University of Cádiz, Spain, discuss their findings on Winogradsky columns they constructed!

If you want to add a post, please feel free to contact the blog administrators (Hera Karayanni, Sokratis Papaspyrou or Kostas Kormas)!

Καλωσορίσατε στη σελίδα των Winobloggers! Διαδικτυακός τόπος συνάντησης φοιτητών, φοιτητριών και διδασκόντων δύο Τμημάτων από την Ελλάδα: Tμήμα Βιολογικών Εφαρμογών και Τεχνολογιών, Παν/μιο Ιωαννίνων και Τμήμα Γεωπονίας, Ιχθυολογίας και Υδάτινου Περιβάλλοντος, Παν/μιο Θεσσαλίας και ενός από την Ισπανία: Σχολή Θετικών Επιστημών, Πανεπιστήμιο του Cadiz. Παρακολουθούμε, σχολιάζουμε, ρωτάμε, απαντάμε σχετικά με τα πειράματά μας, τις στήλες Winogradsky!

Bienvenidos a la pagina web de los Winobloggers! Aquí los estudiantes y profesores de dos departamentos griegos, el Departamento de Aplicaciones y Tecnologías Biológicas de la Universidad de Ioannina y el Departmento de Agricultura, Ictiología y Sistemas Acuáticos de la Universidad de Thessalia, junto con los estudiantes de Microbiología de la Facultad de Ciencias en la Universidad de Cádiz, se reúnen para observar, comentar, preguntar y responder a preguntas relacionadas con nuestro experimento, la columna Winogradsky.

Winogradksy columns

Winogradksy columns
'In the field of observation, chance only favors the prepared mind' Pasteur 1854

Blog posts

Wednesday, 27 May 2015

Aska Ioanna - Giannakos Kostas

Hello everyone!!
            We are a team of two people and we have an experiment about the Winogradsky column. The Winogradsky column is a simple device for culturing a large diversity of microorganisms. The device is a column of sea sediment and water mixed with a carbon source such as newspaper (containing cellulose), egg-shells (containing calcium carbonate), and a sulfur source such as egg yolk. The column is a plastic bottle that is filled with two third full of a rough mixture of ingredients and one third full of sea water.  The place that we put our bottle , was interior. Incubation took place in sunlight for 7 weeks. The ingredients of our experiment were taken from Anauros beach which is located in the southewest of Volos. Let’s see the progress  of our experiment.
1st day
            During the first day of our experiment, the column was sealed and placed in a bright place with small temperature changes.
Image 1

After 2 weeks
            Here we noticed an increase in the turbidity of water. Because of oxygen's low solubility in water, the water quickly becomes anoxic so we observed the creation of a black banding around the bottle. The black color is caused by the presence of FeS (silica-mineral), which is formed anaerobically from the iron and sulfur reactions, as there is production of hydrogen sulfide.
Image 2
After 4 weeks
            Here, the black color increased in our experiment and the  turbidity of water increased too. Anaerobic bacteria are still present to a large extent in the sediment, and there is still capacity for biofilm creation and colony expansion.
Image 3
After 6 weeks
            After 6 weeks we observed that the bottle of our experiment   became  totally  black at all levels and the turbidity reduced in addition with the previous  observation.
Image 4

            While the Winogradsky column is an excellent tool to see whole communities of bacteria, it does not allow one to see the densities or individual bacterial colonies. However its importance in environmental microbiology should not be overlooked and it is still an excellent tool to determine the major bacterial communities in a sample.

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