Winogradsky column lab page!

Welcome to the Winogradsky column lab page! Students from the Departments of Biological Applications and Technology, University of Ioannina and Icthyology and Aquatic Environment, University of Thessaly, Greece and the Microbiology course, Faculty of Sciences, University of Cádiz, Spain, discuss their findings on Winogradsky columns they constructed!

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Καλωσορίσατε στη σελίδα των Winobloggers! Διαδικτυακός τόπος συνάντησης φοιτητών, φοιτητριών και διδασκόντων δύο Τμημάτων από την Ελλάδα: Tμήμα Βιολογικών Εφαρμογών και Τεχνολογιών, Παν/μιο Ιωαννίνων και Τμήμα Γεωπονίας, Ιχθυολογίας και Υδάτινου Περιβάλλοντος, Παν/μιο Θεσσαλίας και ενός από την Ισπανία: Σχολή Θετικών Επιστημών, Πανεπιστήμιο του Cadiz. Παρακολουθούμε, σχολιάζουμε, ρωτάμε, απαντάμε σχετικά με τα πειράματά μας, τις στήλες Winogradsky!

Bienvenidos a la pagina web de los Winobloggers! Aquí los estudiantes y profesores de dos departamentos griegos, el Departamento de Aplicaciones y Tecnologías Biológicas de la Universidad de Ioannina y el Departmento de Agricultura, Ictiología y Sistemas Acuáticos de la Universidad de Thessalia, junto con los estudiantes de Microbiología de la Facultad de Ciencias en la Universidad de Cádiz, se reúnen para observar, comentar, preguntar y responder a preguntas relacionadas con nuestro experimento, la columna Winogradsky.

Winogradksy columns

Winogradksy columns
'In the field of observation, chance only favors the prepared mind' Pasteur 1854

Blog posts

Wednesday, 26 October 2016

                        DIMITRA-IOLI SKOUROLIAKOU
Our experiment took place on 21/10/16 in Vrellis lake of Ioannina at about 1.00 p.m. For our sampling, we used two plastic bottles with capacity 1.5L each, which we filled up with mud and water from the lake. For both bottles we followed the same procedure.
 Except the 2 plastic bottles, we used 2 shovels, a bowl and of course… our hands, so we could collect the proper amount of mud we needed. In order to create an enriched sludge we used 2 raw eggs (with the yolks) as a source of Sulfur, 4 sheets of newspaper as a source of Carbon, and grass which was consisted in the mud. The mixing happened with our bare hands (it felt like we were 4 again). This enriched sludge, which was used as a substrate, consisted the 2/8 of each bottle. Also, the 5/8 consisted of non-enriched sludge and the rest 1/8 consisted of the lake water (approximately 0,3L).
  First of all, we added the enriched sludge and then we filled it up with the plane mud. After a while, we added the lake water. Finally, we covered the top of the bottle with cellophane, in order to achieve the long-term anoxic conditions.
  As a result, we create two Winogradsky columns, which we expose in two different conditions. The one, is near a window in the Lab and the other one is near to a convertor (40oC). It is important to point out that the second column is near to the conservator for 12 hours and the rest of the day is in standard conditions.

  These pictures refer to the first day of the experiment.
1 Control column         

2 warm-exposed column

 Hypothesis: The rapid fluctuation of the temperature on daily basis, reduces the growth rate of the microorganism in the 2nd Winogradsky column.

1 comment:

  1. Near the mean at your place? This is why I counted one less column?