Winogradsky column lab page!

Welcome to the Winogradsky column lab page! Students from the Departments of Biological Applications and Technology, University of Ioannina and Icthyology and Aquatic Environment, University of Thessaly, Greece and the Microbiology course, Faculty of Sciences, University of Cádiz, Spain, discuss their findings on Winogradsky columns they constructed!

If you want to add a post, please feel free to contact the blog administrators (Hera Karayanni, Sokratis Papaspyrou or Kostas Kormas)!

Καλωσορίσατε στη σελίδα των Winobloggers! Διαδικτυακός τόπος συνάντησης φοιτητών, φοιτητριών και διδασκόντων δύο Τμημάτων από την Ελλάδα: Tμήμα Βιολογικών Εφαρμογών και Τεχνολογιών, Παν/μιο Ιωαννίνων και Τμήμα Γεωπονίας, Ιχθυολογίας και Υδάτινου Περιβάλλοντος, Παν/μιο Θεσσαλίας και ενός από την Ισπανία: Σχολή Θετικών Επιστημών, Πανεπιστήμιο του Cadiz. Παρακολουθούμε, σχολιάζουμε, ρωτάμε, απαντάμε σχετικά με τα πειράματά μας, τις στήλες Winogradsky!

Bienvenidos a la pagina web de los Winobloggers! Aquí los estudiantes y profesores de dos departamentos griegos, el Departamento de Aplicaciones y Tecnologías Biológicas de la Universidad de Ioannina y el Departmento de Agricultura, Ictiología y Sistemas Acuáticos de la Universidad de Thessalia, junto con los estudiantes de Microbiología de la Facultad de Ciencias en la Universidad de Cádiz, se reúnen para observar, comentar, preguntar y responder a preguntas relacionadas con nuestro experimento, la columna Winogradsky.

Winogradksy columns

Winogradksy columns
'In the field of observation, chance only favors the prepared mind' Pasteur 1854

Blog posts

Tuesday, 6 December 2016



Team 7: Serasidis Konstantinos, Tsinoglou Makrina
Week: 4

Fourth week’s observation: 18/11/2016

Between the previous weeks and this week’s observations several changes have been noticed in the Control column. Firstly, at the bottom of the column green color has been noticed. Specifically, these changes state the growth of green sulfur bacteria. These bacteria require anaerobic conditions, sulfide ions and light effect in order to photosynthesize. Also, at the bottom of the column are noticeable black spots that are caused by anaerobic sulfur-reducing bacteria. These bacteria use either sulfate or oxidized forms of sulfur as the terminal electron acceptor, generating large amounts of H2S. The H2S will react with any iron in the sediment, producing black ferrous sulfide. At a higher level of the column a brown color in the sediment shows the growth of heterotrophic bacteria. There is a green color band immediately above the sediment. The green colored band is caused by the diffusion of H2S from the sediment into the water column that enables anaerobic photosynthetic bacteria to grow. The orange color in the water of the column that is caused of purple non-sulfur bacteria seems to be the same as the previous week. Finally, biofilm is observed at the top of the water column. A biofilm is any group of microorganisms in which cells stick to each other and often these cells adhere to a surface.
At the bottom of the column in the dark, the sediment is observed darker in comparison with the previous week. The darker color of the sediment indicates the increasing growth of anaerobic bacteria. Also, black spots are noticed at the lower levels of the sediment that are caused by anaerobic sulfur-reducing bacteria. Additionally, the black band which formed at the upper levels of the sediment is caused by the growth anaerobic sulfur-reducing bacteria. The anaerobic conditions are formed by the diffusion of H2S from the sediment into the water column. Finally, biofilm is observed at the top of the water column.

2.Column in the dark 

1. Control column    

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