Winogradsky column lab page!

Welcome to the Winogradsky column lab page! Students from the Departments of Biological Applications and Technology, University of Ioannina and Icthyology and Aquatic Environment, University of Thessaly, Greece and the Microbiology course, Faculty of Sciences, University of Cádiz, Spain, discuss their findings on Winogradsky columns they constructed!

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Καλωσορίσατε στη σελίδα των Winobloggers! Διαδικτυακός τόπος συνάντησης φοιτητών, φοιτητριών και διδασκόντων δύο Τμημάτων από την Ελλάδα: Tμήμα Βιολογικών Εφαρμογών και Τεχνολογιών, Παν/μιο Ιωαννίνων και Τμήμα Γεωπονίας, Ιχθυολογίας και Υδάτινου Περιβάλλοντος, Παν/μιο Θεσσαλίας και ενός από την Ισπανία: Σχολή Θετικών Επιστημών, Πανεπιστήμιο του Cadiz. Παρακολουθούμε, σχολιάζουμε, ρωτάμε, απαντάμε σχετικά με τα πειράματά μας, τις στήλες Winogradsky!

Bienvenidos a la pagina web de los Winobloggers! Aquí los estudiantes y profesores de dos departamentos griegos, el Departamento de Aplicaciones y Tecnologías Biológicas de la Universidad de Ioannina y el Departmento de Agricultura, Ictiología y Sistemas Acuáticos de la Universidad de Thessalia, junto con los estudiantes de Microbiología de la Facultad de Ciencias en la Universidad de Cádiz, se reúnen para observar, comentar, preguntar y responder a preguntas relacionadas con nuestro experimento, la columna Winogradsky.

Winogradksy columns

Winogradksy columns
'In the field of observation, chance only favors the prepared mind' Pasteur 1854

Blog posts

Saturday, 30 December 2017

High-salinity Winogradsky column, 2017

Students: Niki Chondrelli, Steven Moschos 
Department of Biological Applications and Technologies, University of Ioannina

We constructed two Winogradsky columns with mud and water from the lagoon in Koronisia, in 20th October 2017. We used newspaper as a carbon source and egg as a source of sulfur and carbonate ions. In one column we also added 50gr of salt to check how it would affect the growth of microorganisms. 
We expect a delay in the bacterial growth in the salt-enriched column but similar colour patterns.

Our hypothesis is based on the fact that in every broad taxonomic group there are halophilic species, therefore no significant difference will be observed in the colour patterns. Possibly the diversity of the high-salinity column will be more limited, but this can not be deduced with simple observation, unless a group is completely absent. Also, due to the increased salinity, the number of microorganisms that survive and consequently grow in this column will be lower compared to the standard and therefore the formation of colonies will be delayed.

Our hypothesis was rejected, since we observed the same rates of bacterial growth in both columns. This could be due to the fact that the mud we collected exhibited high salinity (about 80) so halophilic species were already abundant in it, and the addition of salt didn't significantly change their growth conditions.

As it can be seen in the pictures that follow, the colour patterns are similar in both columns throughout the experiment but the colour of the water differs (reddish in the standard column and muddy in the salt-enriched one).

Standard, 26/11/17

Salt, 26/11/17

Standard, 23/11/17
Salt, 23/11/17
Standard, 14/12/17
Salt, 14/12/17

1 comment:

  1. Did you measure/estimate salinity after addition of NaCl?