Winogradsky column lab page!


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Welcome to the Winogradsky column lab page! Students from the Departments of Biological Applications and Technology, University of Ioannina and Icthyology and Aquatic Environment, University of Thessaly, Greece and the Microbiology course, Faculty of Sciences, University of Cádiz, Spain, discuss their findings on Winogradsky columns they constructed!

If you want to add a post, please feel free to contact the blog administrators (Hera Karayanni, Sokratis Papaspyrou or Kostas Kormas)!



Καλωσορίσατε στη σελίδα των Winobloggers! Διαδικτυακός τόπος συνάντησης φοιτητών, φοιτητριών και διδασκόντων δύο Τμημάτων από την Ελλάδα: Tμήμα Βιολογικών Εφαρμογών και Τεχνολογιών, Παν/μιο Ιωαννίνων και Τμήμα Γεωπονίας, Ιχθυολογίας και Υδάτινου Περιβάλλοντος, Παν/μιο Θεσσαλίας και ενός από την Ισπανία: Σχολή Θετικών Επιστημών, Πανεπιστήμιο του Cadiz. Παρακολουθούμε, σχολιάζουμε, ρωτάμε, απαντάμε σχετικά με τα πειράματά μας, τις στήλες Winogradsky!


Bienvenidos a la pagina web de los Winobloggers! Aquí los estudiantes y profesores de dos departamentos griegos, el Departamento de Aplicaciones y Tecnologías Biológicas de la Universidad de Ioannina y el Departmento de Agricultura, Ictiología y Sistemas Acuáticos de la Universidad de Thessalia, junto con los estudiantes de Microbiología de la Facultad de Ciencias en la Universidad de Cádiz, se reúnen para observar, comentar, preguntar y responder a preguntas relacionadas con nuestro experimento, la columna Winogradsky.


Winogradksy columns

Winogradksy columns
'In the field of observation, chance only favors the prepared mind' Pasteur 1854

Blog posts

Saturday, 9 June 2018

UCA_A1, A2: results


Our column was saturated with a lot of nutrients that may have done a bad effect on some microorganisms. We have thought the hypothesis that these microorganism can´t grow because of this saturation that have made microorganisms produce second metabolites which have changed pH's environment and maybe that is the reason why some microorganisms died. Also, de high concentration of glucose inhibited some reactions that could be necessary to the microorganisms. On the other hand, microorganisms that were able to live in this condition are the ones which we mentioned in the last post. These are anaerobe microorganisms who produce sulfhydryl acid which precipitates as iron sulphide II (black) and the acid mentioned is the bubble we see on the top.


On the other hand, the column A1 presents the variety of microorganism that we expect. We have to differentiate two zones, the anaerobic and the aerobic:

In the aerobic zone we can see many different types of colonies. These microorganisms usually inhabit in aquatic zone as we can relate in the photos. If they are phototroph they should have a Green coloration, that´s because they have chlorophyll pigments in their chloroplasts. As we can relate in the picture our Winograd sky column also present purple/Brown colonies of phototrophic bacteria’s, they have that colour because they don´t present any chlorophyll pigments.


In the anaerobic zone we discover the presence of sulphate reductors, that kind of microorganism have black pigmentation and are named chemotrophic. We can´t see any other relevant colonies, our theory is that the capacity of reproduction in that organism is better than the others one.

In conclusion the theories that we propose on the first days of class, was not confirmed. We talk about the presence of lactic oxidators but instead we can only observe the most habitual microorganism.




Apart from that we we´re observing the increase of many microorganism likewise we also see the difference between the strata that appears in the column and how they distribute making a microecosystem.

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