Winogradsky column lab page!

Welcome to the Winogradsky column lab page! Students from the Departments of Biological Applications and Technology, University of Ioannina and Icthyology and Aquatic Environment, University of Thessaly, Greece and the Microbiology course, Faculty of Sciences, University of Cádiz, Spain, discuss their findings on Winogradsky columns they constructed!

If you want to add a post, please feel free to contact the blog administrators (Hera Karayanni, Sokratis Papaspyrou or Kostas Kormas)!

Καλωσορίσατε στη σελίδα των Winobloggers! Διαδικτυακός τόπος συνάντησης φοιτητών, φοιτητριών και διδασκόντων δύο Τμημάτων από την Ελλάδα: Tμήμα Βιολογικών Εφαρμογών και Τεχνολογιών, Παν/μιο Ιωαννίνων και Τμήμα Γεωπονίας, Ιχθυολογίας και Υδάτινου Περιβάλλοντος, Παν/μιο Θεσσαλίας και ενός από την Ισπανία: Σχολή Θετικών Επιστημών, Πανεπιστήμιο του Cadiz. Παρακολουθούμε, σχολιάζουμε, ρωτάμε, απαντάμε σχετικά με τα πειράματά μας, τις στήλες Winogradsky!

Bienvenidos a la pagina web de los Winobloggers! Aquí los estudiantes y profesores de dos departamentos griegos, el Departamento de Aplicaciones y Tecnologías Biológicas de la Universidad de Ioannina y el Departmento de Agricultura, Ictiología y Sistemas Acuáticos de la Universidad de Thessalia, junto con los estudiantes de Microbiología de la Facultad de Ciencias en la Universidad de Cádiz, se reúnen para observar, comentar, preguntar y responder a preguntas relacionadas con nuestro experimento, la columna Winogradsky.

Winogradksy columns

Winogradksy columns
'In the field of observation, chance only favors the prepared mind' Pasteur 1854

Blog posts

Sunday, 15 March 2015

Cadiz, 6B: Winogradsky column: First week (Group 6B)

Hello winobloggers,
We apologize for the delay. This is our first post about the Winogradsky column. We are other three students of biotechnology in the University of cádiz.

We made our Winogradsky column on tuesday, 3th March in our Microbiology laboratory. Our column contains a mixture of muddy sediment from San Pedro river and muddy sediment from a hypersaline pond. Moreover, we added as enrichments : CaCO3, FeSO4 and NaCl(salt).

First, we mixed  the sediment from the river and from the pond in a bucket.We put more sediment from the river and we mixed with our hands. Then, we added the enrichment (one teaspoon of each compounds) and we kneaded again. After that, we introduced the mix in a bottle (around two thirds) and finally, we poured 2 cm of water from the same river.
Our column is situated in a desk next to a window, where receives  the sunlight only on one side.
The column has changed much in this week. The first day it has a grey colouration, the water was turbid and it had two worms. However the tuesday, 10th march, the water was yellowish, the column was dark grey with a reddish colouration on the side where receives the sunlight and black spots have appeared.

We think that  in our Winogradsky column are living or will appear: cyanobacterias in the water (the aerobic zone);purple and green sulfer bacterias in the middle; sulfate-reducing bacteria in the bottom because we added FeSO4 and these bacterias producing a nauseating smell and the black spots that we can see; and ,maybe, halophile bacterias because we added salt. In summary, we think that in the future our Winogradsky column will probably be similar to the theoretical image.

Here you can see the images of our column:



  1. So in just a week, we have a black, a green and a red column! See if you can beat that Volos! :-)

    Where does the red colour come from?

    You also added CaCO3. How would it affect the column?


  2. It is not well appreciated in the photos , but comparing both sides of the bottle was obvious the reddish color of the illuminated side.

    We are not sure about what's the bacteria that causes coppery color. According to the information we have found, it may be red not dependent on sulfur bacteria (Rhodospirillum) or sulfur-dependent bacteria (Chromatium).

    The CaCO3 is a pH regulating agent. This maintains the pH optimum for bacterial survival.