Winogradsky column lab page!

Welcome to the Winogradsky column lab page! Students from the Departments of Biological Applications and Technology, University of Ioannina and Icthyology and Aquatic Environment, University of Thessaly, Greece and the Microbiology course, Faculty of Sciences, University of Cádiz, Spain, discuss their findings on Winogradsky columns they constructed!

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Καλωσορίσατε στη σελίδα των Winobloggers! Διαδικτυακός τόπος συνάντησης φοιτητών, φοιτητριών και διδασκόντων δύο Τμημάτων από την Ελλάδα: Tμήμα Βιολογικών Εφαρμογών και Τεχνολογιών, Παν/μιο Ιωαννίνων και Τμήμα Γεωπονίας, Ιχθυολογίας και Υδάτινου Περιβάλλοντος, Παν/μιο Θεσσαλίας και ενός από την Ισπανία: Σχολή Θετικών Επιστημών, Πανεπιστήμιο του Cadiz. Παρακολουθούμε, σχολιάζουμε, ρωτάμε, απαντάμε σχετικά με τα πειράματά μας, τις στήλες Winogradsky!

Bienvenidos a la pagina web de los Winobloggers! Aquí los estudiantes y profesores de dos departamentos griegos, el Departamento de Aplicaciones y Tecnologías Biológicas de la Universidad de Ioannina y el Departmento de Agricultura, Ictiología y Sistemas Acuáticos de la Universidad de Thessalia, junto con los estudiantes de Microbiología de la Facultad de Ciencias en la Universidad de Cádiz, se reúnen para observar, comentar, preguntar y responder a preguntas relacionadas con nuestro experimento, la columna Winogradsky.

Winogradksy columns

Winogradksy columns
'In the field of observation, chance only favors the prepared mind' Pasteur 1854

Blog posts

Thursday, 12 March 2015


    Hello Winoblog, we are three students from Cádiz University and this is the first post we publish about our Winograndsky column.
    We have used sandy beach sediment from Río San Pedro beach. The column was created on Wednesday, 4th March, in Science Faculty laboratory.
    It has been added material enrichment such as calcium sulfate, calcium carbonate, filter paper cut into pieces (cellulose), and sodium chloride (salt).
    Firstly, it has been added sandy beach sediment on a bucket. Then, it has been added the material enrichment mentioned before and meanwhile we were mixing at the same time. Before the mixture was introduced in the Aquarius bottle (Winograndsky column), it had been added a little bit of watter from the same beach as the sediment, so that the mixture got more liquid than before. Finally, once the mixture was introduced in the bottle, it was added two centimetres more of watter.
The sediments take up 0.8 dm3 of the bottle.
    The column is located next to a window. The sun warms up one side of the column and the other side doesn't receive the sun light because it is next to a wall.
    If we analyze the progress reached in the first week, our bottle contains black points spread out uniformly. We notice black lines in the part which the sun warms up. There are bigger black points which are probably colonies formed by bacterias. There are some dark spots (they're grey) too. That spots are more numerous and bigger in the dark side of the bottle. The last phenomenon we can observe is that, in the surface, there are tiny shells sticked to the bottle.
    The scientific hypothesis is that it will probably appear purple and green bacterias (due to we have added sulphates), cyanobacterias and seaweeds in the surface of the bottle (due to they're aerobic bacterias), and grey and dark grey bacterias (sulfur-reducing bacterias and glucose fermenting bacteria, respectively).

    You can see the results on the next images: 

(This is the place where the column is)



  1. Nicely done. The shells are probably animals that were in the sediment and have died. Is the sediment surface black?
    Does anyone have any idea what these back spots are? It is not due to dark grey or grey bacteria. ;-)
    In your opinion, could the light affect the formation of the black spots?

    1. No, the surface isn't black, it's brown and there are some substances floating on it.
      We think that black spots are fungus, or maybe they are bacterias that will become purple in the future. It isn't affected by the light because there are black spots all over the bottle.