Winogradsky column lab page!

Welcome to the Winogradsky column lab page! Students from the Departments of Biological Applications and Technology, University of Ioannina and Icthyology and Aquatic Environment, University of Thessaly, Greece and the Microbiology course, Faculty of Sciences, University of Cádiz, Spain, discuss their findings on Winogradsky columns they constructed!

If you want to add a post, please feel free to contact the blog administrators (Hera Karayanni, Sokratis Papaspyrou or Kostas Kormas)!

Καλωσορίσατε στη σελίδα των Winobloggers! Διαδικτυακός τόπος συνάντησης φοιτητών, φοιτητριών και διδασκόντων δύο Τμημάτων από την Ελλάδα: Tμήμα Βιολογικών Εφαρμογών και Τεχνολογιών, Παν/μιο Ιωαννίνων και Τμήμα Γεωπονίας, Ιχθυολογίας και Υδάτινου Περιβάλλοντος, Παν/μιο Θεσσαλίας και ενός από την Ισπανία: Σχολή Θετικών Επιστημών, Πανεπιστήμιο του Cadiz. Παρακολουθούμε, σχολιάζουμε, ρωτάμε, απαντάμε σχετικά με τα πειράματά μας, τις στήλες Winogradsky!

Bienvenidos a la pagina web de los Winobloggers! Aquí los estudiantes y profesores de dos departamentos griegos, el Departamento de Aplicaciones y Tecnologías Biológicas de la Universidad de Ioannina y el Departmento de Agricultura, Ictiología y Sistemas Acuáticos de la Universidad de Thessalia, junto con los estudiantes de Microbiología de la Facultad de Ciencias en la Universidad de Cádiz, se reúnen para observar, comentar, preguntar y responder a preguntas relacionadas con nuestro experimento, la columna Winogradsky.

Winogradksy columns

Winogradksy columns
'In the field of observation, chance only favors the prepared mind' Pasteur 1854

Blog posts

Saturday, 21 March 2015


Hi everyone!

This is our first post about Winogradsky Column. We are three students of 1st Biotechnology of the University of Cádiz (UCA), Group A2.

Firstly, we are going to write the “ingredients” which we poured into our bottle on 4th March 2015

-We took sand from Río San Pedro’s beach ( A river that flows next to our university ).
-After diluting in water and mixing, added some pieces of filter paper (which contains cellulose).
-Moreover, Calcium carbonate (CaCO3) and Iron Sulfate II (FeSO4) were also added.

All those substances were put into an Aquarius bottle, and finally, 3 or 4 cm of water were added above the sediment.

The first week, one of our partners carried the column to her home and it underwent some changes while it was in a room where was hit by sunlight on one side for 5 hours per day.

 Day 2 (6th March): Little worms have grown on the surface and some dark spots appears in the not sun-exposed area.

Day 3 (7th March): Worms have passed away and the dark spots remain in the not sun-exposed zone. Nevertheles, some orange filaments start to emerge. We consider that filaments are produced by purple non-sulphur photoheterotrophs.

Day 5 (9th March): Black spots keep in both sides of the bottle and the orange filaments continue growing up.

Day 8 (12th March): After a week of the experiment, there have appeared some shells on the sediment surface. These shells are due to the calcium carbonate we added. However, when it ran out, the shells couldn’t continue growing and had to pass out.

After that week, another member of our group received the column and it was placed in the corner of a courtyard. However, there have been rainy days and the column hasn’t experiment changes apparently. We believe that this could be because of the low temperatures and the lack of sun light. Moreover the air humidity has increased his level and this could be another factor by which there aren’t visible organisms in our Winogradsky column.

Day 12 (16th March) The liquid phase has turned into a intense orange due to the iron from the iron sulfate and the orange filaments are disappearing at the same time the shells die.

Day 16 (20th March) This is the ending of the third week since we created the column and the intense orange isn’t as intense as the 12th day. Besides, the orange strands have disappeared completely. In contrast, algae and several aerobic bacteria live above the water.

Hope to keep on posting soon.

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