Winogradsky column lab page!

Welcome to the Winogradsky column lab page! Students from the Departments of Biological Applications and Technology, University of Ioannina and Icthyology and Aquatic Environment, University of Thessaly, Greece and the Microbiology course, Faculty of Sciences, University of Cádiz, Spain, discuss their findings on Winogradsky columns they constructed!

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Καλωσορίσατε στη σελίδα των Winobloggers! Διαδικτυακός τόπος συνάντησης φοιτητών, φοιτητριών και διδασκόντων δύο Τμημάτων από την Ελλάδα: Tμήμα Βιολογικών Εφαρμογών και Τεχνολογιών, Παν/μιο Ιωαννίνων και Τμήμα Γεωπονίας, Ιχθυολογίας και Υδάτινου Περιβάλλοντος, Παν/μιο Θεσσαλίας και ενός από την Ισπανία: Σχολή Θετικών Επιστημών, Πανεπιστήμιο του Cadiz. Παρακολουθούμε, σχολιάζουμε, ρωτάμε, απαντάμε σχετικά με τα πειράματά μας, τις στήλες Winogradsky!

Bienvenidos a la pagina web de los Winobloggers! Aquí los estudiantes y profesores de dos departamentos griegos, el Departamento de Aplicaciones y Tecnologías Biológicas de la Universidad de Ioannina y el Departmento de Agricultura, Ictiología y Sistemas Acuáticos de la Universidad de Thessalia, junto con los estudiantes de Microbiología de la Facultad de Ciencias en la Universidad de Cádiz, se reúnen para observar, comentar, preguntar y responder a preguntas relacionadas con nuestro experimento, la columna Winogradsky.

Winogradksy columns

Winogradksy columns
'In the field of observation, chance only favors the prepared mind' Pasteur 1854

Blog posts

Sunday, 23 April 2017

UCA_4A_Entrada 1: 1º day

Hi there! We are Elena, Pepi and Marta and we are going to explain how we did our column in the lab. First of all, we mixed  20,9 g of mud with some seawater until we had an homogenean mixture. Then, we added :
- 0,41 g of CaSO4 because it is a source of sulfur for  aminoacids. SO42-  + O.M  à  CO2 + H2S-
- 0,21 g of NaCl because we want halophilic bacteria to grow.
- 0,58 g of CaCOso autotrophic bacteria can grow.
- 0,31 g of C12H22O11, which is a common carbon natural source and also allows heterotrophic microorganisms to grow.
- 0,22 g of cellulose, another carbon natural source that also allows heterotrophic microorganisms to grow.
- 0,1 g of agar, a polysaccharide  that attatches cells.
Now we introduced the mixture in our test tube.
After that, we measured 30 g of mud and we mixed it with 90 g of sand, and we added this new mixture to the test tube too.
We also added a bit of seawater at the top of the test tube and left it in the lab for one week.
The results we expect to get is a concentration gradient that shows the different types of microorganisms that grows in our Winogradsky Column.

1 comment:

  1. Cellulose cannot be used directly by heterotrophs. Who has to intervene?
    Can you explain a bit better why you added agar? Which microorganisms can degrade agar?