Winogradsky column lab page!

Welcome to the Winogradsky column lab page! Students from the Departments of Biological Applications and Technology, University of Ioannina and Icthyology and Aquatic Environment, University of Thessaly, Greece and the Microbiology course, Faculty of Sciences, University of Cádiz, Spain, discuss their findings on Winogradsky columns they constructed!

If you want to add a post, please feel free to contact the blog administrators (Hera Karayanni, Sokratis Papaspyrou or Kostas Kormas)!

Καλωσορίσατε στη σελίδα των Winobloggers! Διαδικτυακός τόπος συνάντησης φοιτητών, φοιτητριών και διδασκόντων δύο Τμημάτων από την Ελλάδα: Tμήμα Βιολογικών Εφαρμογών και Τεχνολογιών, Παν/μιο Ιωαννίνων και Τμήμα Γεωπονίας, Ιχθυολογίας και Υδάτινου Περιβάλλοντος, Παν/μιο Θεσσαλίας και ενός από την Ισπανία: Σχολή Θετικών Επιστημών, Πανεπιστήμιο του Cadiz. Παρακολουθούμε, σχολιάζουμε, ρωτάμε, απαντάμε σχετικά με τα πειράματά μας, τις στήλες Winogradsky!

Bienvenidos a la pagina web de los Winobloggers! Aquí los estudiantes y profesores de dos departamentos griegos, el Departamento de Aplicaciones y Tecnologías Biológicas de la Universidad de Ioannina y el Departmento de Agricultura, Ictiología y Sistemas Acuáticos de la Universidad de Thessalia, junto con los estudiantes de Microbiología de la Facultad de Ciencias en la Universidad de Cádiz, se reúnen para observar, comentar, preguntar y responder a preguntas relacionadas con nuestro experimento, la columna Winogradsky.

Winogradksy columns

Winogradksy columns
'In the field of observation, chance only favors the prepared mind' Pasteur 1854

Blog posts

Thursday, 20 April 2017

Winoblog, First post, group B7.

          In this post, we are going to explain our theories about the experiment that we have done in microbiology. This experiment is called “Winogradsky column”, his name came from first scientist that realized it.
           Our column was filled up with portions of natural mud, sand, and sea water. These are the column’s principal elements, but we need to add more nutrients for bacterial growth.   We need to create an oxygen gradient. In the bottom of the column there aren’t any oxygen (only sulphates SO42-), and in the top the oxygen concentration is equal to the atmospheric.

         In order to create this gradient, we putted several nutrients to feed diferent kinds of bacterias. It is use to occasion a microecosystem where there are many food chains.
The nutrients we added were:

  • -FeSO4 (to give a sulphate source, and a iron source).
  • -Cellulose (to give a source of carbohydrates that some kind of bacterias can use).
  • -NaCl  (to equilibrate column’s osmotic pressure).
  • -CaSO4, Chalk (in order to give a calcium and sulphate source).
  • -Yeast extract (it is use to nutrient source).

          Amount nutrients can change according to microorganism’s needs. It preferably utilize a low concentation of those because our Winogradsky column can take a long time to flourish o it can lead to methane wall cavities.

Figure 1: Methane wall cavities.
Figure 2: Winogradsky column. Photo taken after one week.

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