Winogradsky column lab page!


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Welcome to the Winogradsky column lab page! Students from the Departments of Biological Applications and Technology, University of Ioannina and Icthyology and Aquatic Environment, University of Thessaly, Greece and the Microbiology course, Faculty of Sciences, University of Cádiz, Spain, discuss their findings on Winogradsky columns they constructed!

If you want to add a post, please feel free to contact the blog administrators (Hera Karayanni, Sokratis Papaspyrou or Kostas Kormas)!



Καλωσορίσατε στη σελίδα των Winobloggers! Διαδικτυακός τόπος συνάντησης φοιτητών, φοιτητριών και διδασκόντων δύο Τμημάτων από την Ελλάδα: Tμήμα Βιολογικών Εφαρμογών και Τεχνολογιών, Παν/μιο Ιωαννίνων και Τμήμα Γεωπονίας, Ιχθυολογίας και Υδάτινου Περιβάλλοντος, Παν/μιο Θεσσαλίας και ενός από την Ισπανία: Σχολή Θετικών Επιστημών, Πανεπιστήμιο του Cadiz. Παρακολουθούμε, σχολιάζουμε, ρωτάμε, απαντάμε σχετικά με τα πειράματά μας, τις στήλες Winogradsky!


Bienvenidos a la pagina web de los Winobloggers! Aquí los estudiantes y profesores de dos departamentos griegos, el Departamento de Aplicaciones y Tecnologías Biológicas de la Universidad de Ioannina y el Departmento de Agricultura, Ictiología y Sistemas Acuáticos de la Universidad de Thessalia, junto con los estudiantes de Microbiología de la Facultad de Ciencias en la Universidad de Cádiz, se reúnen para observar, comentar, preguntar y responder a preguntas relacionadas con nuestro experimento, la columna Winogradsky.


Winogradksy columns

Winogradksy columns
'In the field of observation, chance only favors the prepared mind' Pasteur 1854

Blog posts

Friday, 14 February 2020

Winogradsky Column


Vragkalis Georgios
Department of Biological Applications and Technology
University of Ioannina

Hypothesis: We expect to notice green and purple sulfur bacteria in the mud and cyanobacteria in the water.

Sampling Areas: Mud was selected from Kalodiki wetland, while water was selected from Pamvotis lake. Both systems are eutrophic. The weather was sunny and warm without any rain for a long period of time, which caused the wetland to be dried up on some of its regions.

The Column: Mud was selected from the wetland. 2/3 of mud were used to fill the column (1,5L plastic bottle). 1/3 of it was mixed with a whole egg and shredded paper and put at the bottom of the column. Nothing was put on the rest of the mud. Finally, the column was filled with 0,5L of water from Pamvotis lake. The column was maintained in room temperature and exposed to light.

Results: After a week, cyanobacteria were noticed in the mud and in the water. On day 19, purple and green sulfur bacteria were noticed in the anaerobic zone of the column and became more visible on day 26. On day 40, we can see some spots of black pigment at the bottom of the column. Those are sedimentations of iron sulfide II. Though we can’t see them macroscopically, we could also say that sulfate reducing bacteria exist at the bottom of the column.

Below you can see some pictures of the column:







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