Vragkalis Georgios
Department
of Biological Applications and Technology
University
of Ioannina
Hypothesis:
We expect to notice green and purple sulfur bacteria in the mud and
cyanobacteria in the water.
Sampling
Areas: Mud was selected from Kalodiki wetland, while water was
selected from Pamvotis lake. Both systems are eutrophic. The weather
was sunny and warm without any rain for a long period of time, which caused the wetland to be dried up on some of its regions.
The
Column: Mud was selected from
the wetland. 2/3 of mud were used to fill the column (1,5L plastic
bottle). 1/3 of it was mixed with a whole egg and shredded paper and
put at the bottom of the column. Nothing was put on the rest of the
mud. Finally, the column was filled with 0,5L of water from Pamvotis
lake. The column was maintained in room temperature and exposed to
light.
Results:
After a week, cyanobacteria
were noticed in the mud and in the water. On
day 19, purple and green sulfur bacteria were noticed in the
anaerobic zone of the column and became more visible on day 26. On day 40, we can see some spots of
black pigment at the bottom of the column. Those are sedimentations
of iron sulfide II. Though we can’t see them macroscopically, we
could also say that sulfate reducing bacteria exist at the bottom of
the column.
Below
you can see some pictures of the column:
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