UCA_4A_Entrada 1: 1º day

Hi there! We are Elena, Pepi and Marta and we are going to explain how we did our column in the lab. First of all, we mixed  20,9 g of mud with some seawater until we had an homogenean mixture. Then, we added :
- 0,41 g of CaSO₄ because it is a source of sulfur for  aminoacids. SO₄^2-  + O.M  à  CO₂ + H₂S^-

- 0,21 g of NaCl because we want halophilic bacteria to grow.
- 0,58 g of CaCO₃ so autotrophic bacteria can grow.
- 0,31 g of C₁₂H₂₂O₁₁, which is a common carbon natural source and also allows heterotrophic microorganisms to grow.
- 0,22 g of cellulose, another carbon natural source that also allows heterotrophic microorganisms to grow.
- 0,1 g of agar, a polysaccharide  that attatches cells.
Now we introduced the mixture in our test tube.
After that, we measured 30 g of mud and we mixed it with 90 g of sand, and we added this new mixture to the test tube too.
We also added a bit of seawater at the top of the test tube and left it in the lab for one week.
The results we expect to get is a concentration gradient that shows the different types of microorganisms that grows in our Winogradsky Column.