- -FeSO4 (to give a sulphate source, and a ferrum source).
- -Cellulose (to give a source of carbón hidrates that some kind of bacterias can use).
- -NaCl (to equilibrate column’s osmotic pressure).
- -CaSO4, Chalk (in order to give a calcium and sulphate souce).
- -Yeast extract (it is use to nutrient source).
|Figure 1: Methane wall cavities.|
|Figure 2: Winogradsky column. Photo taken after one week.|