UCA_E5-6MICRO: First day.
Hi! We are Anabel, Paco, Irene, Misael and
Gabriel students of Enology. We will show you our experiment of Winogradsky
column during this quarter.
To prepare our Winogradsky’s column
we have chosen supply carbon with extract of yeast due to it is an excellent
way to get nutrients like vitamins and amino acids. We have weighed 1,4g.
Later we have weighed 40g of mud and
0,6g of Na2SO4 which is the substrate to growth of
sulphate reducing bacterias.
The mud and Na2SO4 are
mixed with extract of yeast and they are divided in two columns.
Then we weighed 180g of sand and 60g
of mud, then mixed and divided in columns. One column was put in the dark and
the other one was put in natural light.
What we expect is that there is
stablished oxygen gradient and an interdependence in the columns between
different micro-organisms included in the columns.
In the zone below at columns
micro-organisms that develop fermentatives process should grow producing
alcohol and fatty acids like subproducts of their metabolism. This products are
substrates for the development of sulphate reducing bacterias. This bacterias
liberate sulphide that spread to the oxygenated higher zone creating a gradient
where photosynthetic bacterias that use sulphur are developed.
Finally, the cianobacterias and
algae grow in the higher zone and they liberate oxygen that keep this zone
aerobic.
We haven't taken photos of
our column in this first state, but later we will upload some of them.
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