Hello we are David , Diego and Malena, we are Biotechnology students. We have prepared a Winogradsky column and we are going to show the changes that have been produced.
The Winogradsky column is a microorganism cultivating resource. The base components we use are :
- Sand 75 gr.
-Mud 25 gr.
-Thin layer of sea water
This components are the same for every columns. Moreover we decided to add :
-0.13 gr of sugar as a carbon source
-0.10 gr of calcium sulfate as a sulfur source
- 0.09 gr of yeast to produce fermentation.At the bottom of the column there is no oxygen, so it will be a different source of organic matter,for example , lactic acid.
We will try to make a gradient of O2 at the top and H2S at the bottom so that both aerobic and anaerobic organism can grow.
Our hypothesis is that heterotroph organism will grow because of the external carbon addition and also sulfur fixative bacteria because of the sulfur source.
What do we see in the columns of the pictures? Can you give us an example of a sulfur fixative bacteria?
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