Winogradsky column lab page!

Welcome to the Winogradsky column lab page! Students from the Departments of Biological Applications and Technology, University of Ioannina and Icthyology and Aquatic Environment, University of Thessaly, Greece and the Microbiology course, Faculty of Sciences, University of Cádiz, Spain, discuss their findings on Winogradsky columns they constructed!

If you want to add a post, please feel free to contact the blog administrators (Hera Karayanni, Sokratis Papaspyrou or Kostas Kormas)!

Καλωσορίσατε στη σελίδα των Winobloggers! Διαδικτυακός τόπος συνάντησης φοιτητών, φοιτητριών και διδασκόντων δύο Τμημάτων από την Ελλάδα: Tμήμα Βιολογικών Εφαρμογών και Τεχνολογιών, Παν/μιο Ιωαννίνων και Τμήμα Γεωπονίας, Ιχθυολογίας και Υδάτινου Περιβάλλοντος, Παν/μιο Θεσσαλίας και ενός από την Ισπανία: Σχολή Θετικών Επιστημών, Πανεπιστήμιο του Cadiz. Παρακολουθούμε, σχολιάζουμε, ρωτάμε, απαντάμε σχετικά με τα πειράματά μας, τις στήλες Winogradsky!

Bienvenidos a la pagina web de los Winobloggers! Aquí los estudiantes y profesores de dos departamentos griegos, el Departamento de Aplicaciones y Tecnologías Biológicas de la Universidad de Ioannina y el Departmento de Agricultura, Ictiología y Sistemas Acuáticos de la Universidad de Thessalia, junto con los estudiantes de Microbiología de la Facultad de Ciencias en la Universidad de Cádiz, se reúnen para observar, comentar, preguntar y responder a preguntas relacionadas con nuestro experimento, la columna Winogradsky.

Winogradksy columns

Winogradksy columns
'In the field of observation, chance only favors the prepared mind' Pasteur 1854

Blog posts

Wednesday, 16 March 2016

Winogradsky column, Department of Biological Applications & Technology, Ioannina

Hello everybody,
My name is George Sartzis and I'm an undergraduate student of Biology from the Department of Biological Applications and Technology of the University of Ioannina.
I'm presenting my results from the Winogradsky column experiment that was conducted in 23/10/2015 at Voidomatis River.
I made one column from a 1.5 L plastic bottle which by 1/3 contains mud from the riverside mixed with enrichment materials (1 chicken egg with its shell, 5g of baking powder and some newspaper pieces) and by 2/3 pure riverside mud. After the column was filled to the mouth of the bottle, the mud precipitated and a zone of water was created occupying 25% of the column.
The bottle was put in conditions of medium (to low) light intensity and temperatures of 10 to 15°C.
Today, 3 and a half months after the column was made, outwardly, not much seem to have changed. The mud is a little bit darker and the water became more blurred than the first day.
Although there were (and still are) too few visual indications that "something was going on in there", 2 1/2 months after the creation of the column, the cap of the bottle cracked due to the high pressure of the inside of the bottle. A small quantity of water was spilled out and a strong rotten egg smell arose. 2 days after that incident the spilled water had evaporated leaving behind a red smudge on the floor.
I suppose that sulfur-reducing cells have grown in the mud and they produce H2S. That's why the pressure of the bottle was high before the bottle cap cracked. H2S production also explains the rotten egg smell. As for the red smudge from the spilled water I believe that microorganisms that existed in the water found the conditions outside of the bottle more suitable to increase in numbers and predominate against other species which were dominant in the inside of the bottle.
Furthermore, I think that probably the conditions of the room where the bottle was put were not suitable for most of the cells in the sample to grow and/or the riverside mud's microbial load was quiet low. That's why there are, until now, too few changes in the visual appearance of the column.
Unfortunately I currently have no photos of my Winogradsky column to post. I will upload some photos as soon as possible.
I will make a new post when something changes!

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