Winogradsky column lab page!

Welcome to the Winogradsky column lab page! Students from the Departments of Biological Applications and Technology, University of Ioannina and Icthyology and Aquatic Environment, University of Thessaly, Greece and the Microbiology course, Faculty of Sciences, University of Cádiz, Spain, discuss their findings on Winogradsky columns they constructed!

If you want to add a post, please feel free to contact the blog administrators (Hera Karayanni, Sokratis Papaspyrou or Kostas Kormas)!

Καλωσορίσατε στη σελίδα των Winobloggers! Διαδικτυακός τόπος συνάντησης φοιτητών, φοιτητριών και διδασκόντων δύο Τμημάτων από την Ελλάδα: Tμήμα Βιολογικών Εφαρμογών και Τεχνολογιών, Παν/μιο Ιωαννίνων και Τμήμα Γεωπονίας, Ιχθυολογίας και Υδάτινου Περιβάλλοντος, Παν/μιο Θεσσαλίας και ενός από την Ισπανία: Σχολή Θετικών Επιστημών, Πανεπιστήμιο του Cadiz. Παρακολουθούμε, σχολιάζουμε, ρωτάμε, απαντάμε σχετικά με τα πειράματά μας, τις στήλες Winogradsky!

Bienvenidos a la pagina web de los Winobloggers! Aquí los estudiantes y profesores de dos departamentos griegos, el Departamento de Aplicaciones y Tecnologías Biológicas de la Universidad de Ioannina y el Departmento de Agricultura, Ictiología y Sistemas Acuáticos de la Universidad de Thessalia, junto con los estudiantes de Microbiología de la Facultad de Ciencias en la Universidad de Cádiz, se reúnen para observar, comentar, preguntar y responder a preguntas relacionadas con nuestro experimento, la columna Winogradsky.

Winogradksy columns

Winogradksy columns
'In the field of observation, chance only favors the prepared mind' Pasteur 1854

Blog posts

Friday, 4 November 2016

Team: 4
Tampakidou Maria-Ioanna, 1479
Mpelonias Fivos, 1178

The experiment was constructed at 21/10/2016 in the lake Vrellis, which is a small, seasonal, shallow lake 12 km outside of ioannina city. The goal of this experiment is the construction of a winograsky column. Actually two winograsky columns were constructed  in order to involve two different factors whom any discrapancies could be observed.
·         2 plastic bottles (1,5 L)
·         Mud sediment from the lake
·         Lake water
·         2 eggs
·         Piece of newspaper
·         Plaster
·         Plastic membrane

These columns contains muddy sediment whish was taken from the lake and was mixed with nutricius materials that are full of carbon, soulphate and protein sources. In the first case, mud has been added and mixed with two eggs (protein and soulphate source - buffer factor) and pieces of newspaper (carbon source) in a plastic bottle. In the second case, instead of eggs, has been added plaster (full of sulfur). After the bottled have been half contained with  the nutricious mud, extra mud without nutrients was added so the 2/3 of the bottles were full.  Aferwards, water from the lake was added up immediately.  Moreover, it should be indicated that the second plastic bottle has been cut and coating with plastic membrane

Muds sediment reposit.
At first, the basic hypothesis is the growth of several types of microorganisms such as different kinds of bacteria and phytoplangton . We consider as a contributory factor of the microbes increasement,  the fact that the lake ranging from mesotrophic to eutrophic.  The diversity of microorganisms will be depended on the gradation of the oxygen across the bottle and the presence of soulphates. Therefore, in the top of it will be grown aerobic microorganism such us photosynthetic cyanobacteria and algae, in the middle of the bottle will be grown aerotolerant  microbes that they do not use sulful (there is not any of it in this level) such us non-sulfur photosynthetic bacteria (anaerobic zone: less anaerobic to more anaerobic). At the bottom of the bottle, the presence of totally anaerobic photoautotrophic microbes propably will be observed. These microbes use sulfur ions to produce energy. Microorganisms like the phylum clorobi convert sulphates ions into hydrogen sulfide which afterwards could be used by other anoxic tolerant organisms.
There is one more conjecture in this experiment which is relevant with the difference between the nutrient conditions in the two bottles. More specific, in the first bottle the increase of various microorganisms will be bigger than the second because eggs contains a variety of nutrients  like proteins, fatty acids, minerals.
Winograsky-Column-1 which contains eggs (right) and Winograsky-column-2 which contains plaster (left).

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