Winogradsky column lab page!

Welcome to the Winogradsky column lab page! Students from the Departments of Biological Applications and Technology, University of Ioannina and Icthyology and Aquatic Environment, University of Thessaly, Greece and the Microbiology course, Faculty of Sciences, University of Cádiz, Spain, discuss their findings on Winogradsky columns they constructed!

If you want to add a post, please feel free to contact the blog administrators (Hera Karayanni, Sokratis Papaspyrou or Kostas Kormas)!

Καλωσορίσατε στη σελίδα των Winobloggers! Διαδικτυακός τόπος συνάντησης φοιτητών, φοιτητριών και διδασκόντων δύο Τμημάτων από την Ελλάδα: Tμήμα Βιολογικών Εφαρμογών και Τεχνολογιών, Παν/μιο Ιωαννίνων και Τμήμα Γεωπονίας, Ιχθυολογίας και Υδάτινου Περιβάλλοντος, Παν/μιο Θεσσαλίας και ενός από την Ισπανία: Σχολή Θετικών Επιστημών, Πανεπιστήμιο του Cadiz. Παρακολουθούμε, σχολιάζουμε, ρωτάμε, απαντάμε σχετικά με τα πειράματά μας, τις στήλες Winogradsky!

Bienvenidos a la pagina web de los Winobloggers! Aquí los estudiantes y profesores de dos departamentos griegos, el Departamento de Aplicaciones y Tecnologías Biológicas de la Universidad de Ioannina y el Departmento de Agricultura, Ictiología y Sistemas Acuáticos de la Universidad de Thessalia, junto con los estudiantes de Microbiología de la Facultad de Ciencias en la Universidad de Cádiz, se reúnen para observar, comentar, preguntar y responder a preguntas relacionadas con nuestro experimento, la columna Winogradsky.

Winogradksy columns

Winogradksy columns
'In the field of observation, chance only favors the prepared mind' Pasteur 1854

Blog posts

Wednesday, 16 November 2016

Tampakidou Maria-Ioanna Mpelonias Fivos University of Ioannina

Team 4

Tampakidou Maria-Ioanna, 1479, Mpelonias Fivos, 1178 University of Ioannina

Second week observation:  4/11/2016

There is observable and notable growth in both bottles. The winograsky column-1 (contains eggs and it is closed) on the right has a big difference this week. At first the color of the water in the top of it, is a dark green-black. Moreover, lots of  bubbles started to emerge when the bottle was opened while in the same time an intense smell of methane made us ‘’chocking’’ . This could be a proof  for the existence of methanogen Archae. This kind of microorganism should be present in the lower layer where the conditions are totally anoxic.  Apart from methanogens, probably there are some sulfur chemotrophic or photosynthetic bacteria in this specific layer. These sulfur oxidizers release H2S which could be a contributory factor in the bad smell too. We can also assume that Iron bacteria colonize the transition zone where de-oxygenated water from an anaerobic environment flows into an aerobic environment (upper zone). Lastly there might be some aerobic microorganisms on the top of the column (dark green color) like cyanobacteria. In the winograsky column-2 (contains plasters and it is open) the water is still clear but the mud, in some points, have become totally black. This reveals anoxic conditions and the presence of decomposers inside the column. These microorganisms could be sulfur or non sulfur, photosynthetic or not bacteria.
Despite of column-1, there isn’t any observable difference in the water zone in column-2. This might happen because: First, there is contact between atmosphere and column. Second there is absence of a great number of nutrients (eggs contain and make the conditions more favorable). Therefore the microorganism increasement is higher in the first column. On the other hand, the plaster might contribute in the sulfur bacteria growth. Consequently, these kind of microbes may be dominant in this column and inhibit the development of other microbe species such as methanogens (there is any specific clue about them).