Winogradsky column lab page!

Welcome to the Winogradsky column lab page! Students from the Departments of Biological Applications and Technology, University of Ioannina and Icthyology and Aquatic Environment, University of Thessaly, Greece and the Microbiology course, Faculty of Sciences, University of Cádiz, Spain, discuss their findings on Winogradsky columns they constructed!

If you want to add a post, please feel free to contact the blog administrators (Hera Karayanni, Sokratis Papaspyrou or Kostas Kormas)!

Καλωσορίσατε στη σελίδα των Winobloggers! Διαδικτυακός τόπος συνάντησης φοιτητών, φοιτητριών και διδασκόντων δύο Τμημάτων από την Ελλάδα: Tμήμα Βιολογικών Εφαρμογών και Τεχνολογιών, Παν/μιο Ιωαννίνων και Τμήμα Γεωπονίας, Ιχθυολογίας και Υδάτινου Περιβάλλοντος, Παν/μιο Θεσσαλίας και ενός από την Ισπανία: Σχολή Θετικών Επιστημών, Πανεπιστήμιο του Cadiz. Παρακολουθούμε, σχολιάζουμε, ρωτάμε, απαντάμε σχετικά με τα πειράματά μας, τις στήλες Winogradsky!

Bienvenidos a la pagina web de los Winobloggers! Aquí los estudiantes y profesores de dos departamentos griegos, el Departamento de Aplicaciones y Tecnologías Biológicas de la Universidad de Ioannina y el Departmento de Agricultura, Ictiología y Sistemas Acuáticos de la Universidad de Thessalia, junto con los estudiantes de Microbiología de la Facultad de Ciencias en la Universidad de Cádiz, se reúnen para observar, comentar, preguntar y responder a preguntas relacionadas con nuestro experimento, la columna Winogradsky.

Winogradksy columns

Winogradksy columns
'In the field of observation, chance only favors the prepared mind' Pasteur 1854

Blog posts

Tuesday, 14 April 2015

Cadiz, 9B: Winogradsky column, second, third, fourth and fifth week.

Hi, everyone! I know I should post more often, but the column did not change much...

  • Week 2, 17th March: 
The number of black dots has increased, as I said in the previous post, I think that they are colonies of desulfovibrio bacteria, the foul odour of the bottle has increased.

  • Week 3, 24th March:
The number of black dots has increased(again). There are more desulfovibrio  bacteria.
The water used to be orange, but now it is pale brown.
There aren't black dots near the water, that zone (the less anaerobic zone) is brown.

  • Week 4, 31st March: 
The black colour, which according to our hypothesis are the colonies of desulfovibrio bacteria, prevails in the bottle, but the mud near the water is brown. It is explained by the existence of the oxygen and the sulfur gradient, the desulfovibro bacteria are in the anaerobic zone, where the hydrogen sulfide concentration is higher.
The microaerophilic zone,the interface of sulfide-containing mud and the more aerobic pond water, is brown, we think that bacteria that can oxidise sulfur are growing there, they oxidise it to obtain energy and that energy is used to produce organic molecules.
Under the previous zone, the mud is orange, the presence of iron oxidising bacteria can produce this colouration.
There are some bivalves in the brown mud.
The water, the aerobic zone, is not transparent, photosynthetic bacteria(cyanobacteria) and algae may be growing there. They use light energy and CO2 to produce organic molecules and oxygen, maintaining the O2 gradient.

  • Week 5, 7th April:
The water colour has changed, it is brown, the surface is not transparent, this change might have been produced by the increasing number of cyanobacteria.
The microaerophilic's zone colour is pale brown, the less anaerobic zone colour is brown (rust colour), and orange.
Nonsulfur photosynthetic bacteria grow in the rust coloured zone, they produce organic molecules when they fix CO2 , they use organic acids or ethanol as electron donors rather than sulfide.

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