Winogradsky column lab page!

Welcome to the Winogradsky column lab page! Students from the Departments of Biological Applications and Technology, University of Ioannina and Icthyology and Aquatic Environment, University of Thessaly, Greece and the Microbiology course, Faculty of Sciences, University of Cádiz, Spain, discuss their findings on Winogradsky columns they constructed!

If you want to add a post, please feel free to contact the blog administrators (Hera Karayanni, Sokratis Papaspyrou or Kostas Kormas)!

Καλωσορίσατε στη σελίδα των Winobloggers! Διαδικτυακός τόπος συνάντησης φοιτητών, φοιτητριών και διδασκόντων δύο Τμημάτων από την Ελλάδα: Tμήμα Βιολογικών Εφαρμογών και Τεχνολογιών, Παν/μιο Ιωαννίνων και Τμήμα Γεωπονίας, Ιχθυολογίας και Υδάτινου Περιβάλλοντος, Παν/μιο Θεσσαλίας και ενός από την Ισπανία: Σχολή Θετικών Επιστημών, Πανεπιστήμιο του Cadiz. Παρακολουθούμε, σχολιάζουμε, ρωτάμε, απαντάμε σχετικά με τα πειράματά μας, τις στήλες Winogradsky!

Bienvenidos a la pagina web de los Winobloggers! Aquí los estudiantes y profesores de dos departamentos griegos, el Departamento de Aplicaciones y Tecnologías Biológicas de la Universidad de Ioannina y el Departmento de Agricultura, Ictiología y Sistemas Acuáticos de la Universidad de Thessalia, junto con los estudiantes de Microbiología de la Facultad de Ciencias en la Universidad de Cádiz, se reúnen para observar, comentar, preguntar y responder a preguntas relacionadas con nuestro experimento, la columna Winogradsky.

Winogradksy columns

Winogradksy columns
'In the field of observation, chance only favors the prepared mind' Pasteur 1854

Blog posts

Wednesday, 1 April 2015

Cadiz, 7A: First week (March 4th-11th) Biotechnology A7

Hello everyone!

We are a group of students of Biotechnology from the University of Cadiz.
We made our Winogradsky on March, 4th. The column is composed of some muddy sediment from an hypersaline lagoon, at which we added some enrichment material in order to promote bacteria’s growth:

- Calcium sulphate (CaSO4) and ferrous sulphate (FeSO4): By adding these components we enhance the growth of sulphate-reducing bacteria, as they use sulphate as oxidant agent, reducing it to sulfide. Also these components provide Ca2+ and Fe2+ ions to the column.
- Sodium chloride (NaCl) in small quantities, which provides Na+ and Cl+ ions, necessary for bacteria's metabolism.
- Motor oil, as a source of carbon.

Firstly at the laboratory we placed our sediment in a bucket and cleaned the sediment out of rubbish. Once cleaned, we added the enrichment material and mixed with our hands. The mixture was then filled in a 1,5 L capacity bottle, filling about ½ of its capacity. Finally we added to the bottle some water from the same lagoon, to fill about 2/3 of the bottle. The Winogradsky column was ready to start the experiment!

The bottle was placed in the corner of a well-illuminated courtyard, but not directly in the sun. One side of the bottle is always directed toward the sunlight while the back, where the sunlight doesn’t reach, is directed toward the walls. By this we’ll differentiate bacteria that use sunlight to growth to those that don’t. Moreover, the bottle remained always uncovered, so bacteria can obtain oxygen from the outside. This way we made a gradient of oxygen inside the bottle, where bacteria that use oxygen will grow at the top of the bottle (where oxygen is more abundant) and those that do not use oxygen will grow at the bottom.

This is the appearance of the bottle the second day (March 5th). We can see the turbid water on the top of the column, and the sediment below. The back side of the bottle has exactly the same appearance.

Here is a top view of the bottle:

The first week we've not reported many changes: the sediment became darker, and in the third day some snails from the outside entered the bottle.

We'll keep posting the evolution of our column over the weeks. See you soon!

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