Winogradsky column lab page!

Welcome to the Winogradsky column lab page! Students from the Departments of Biological Applications and Technology, University of Ioannina and Icthyology and Aquatic Environment, University of Thessaly, Greece and the Microbiology course, Faculty of Sciences, University of Cádiz, Spain, discuss their findings on Winogradsky columns they constructed!

If you want to add a post, please feel free to contact the blog administrators (Hera Karayanni, Sokratis Papaspyrou or Kostas Kormas)!

Καλωσορίσατε στη σελίδα των Winobloggers! Διαδικτυακός τόπος συνάντησης φοιτητών, φοιτητριών και διδασκόντων δύο Τμημάτων από την Ελλάδα: Tμήμα Βιολογικών Εφαρμογών και Τεχνολογιών, Παν/μιο Ιωαννίνων και Τμήμα Γεωπονίας, Ιχθυολογίας και Υδάτινου Περιβάλλοντος, Παν/μιο Θεσσαλίας και ενός από την Ισπανία: Σχολή Θετικών Επιστημών, Πανεπιστήμιο του Cadiz. Παρακολουθούμε, σχολιάζουμε, ρωτάμε, απαντάμε σχετικά με τα πειράματά μας, τις στήλες Winogradsky!

Bienvenidos a la pagina web de los Winobloggers! Aquí los estudiantes y profesores de dos departamentos griegos, el Departamento de Aplicaciones y Tecnologías Biológicas de la Universidad de Ioannina y el Departmento de Agricultura, Ictiología y Sistemas Acuáticos de la Universidad de Thessalia, junto con los estudiantes de Microbiología de la Facultad de Ciencias en la Universidad de Cádiz, se reúnen para observar, comentar, preguntar y responder a preguntas relacionadas con nuestro experimento, la columna Winogradsky.

Winogradksy columns

Winogradksy columns
'In the field of observation, chance only favors the prepared mind' Pasteur 1854

Blog posts

Saturday, 27 May 2017

First week, group 1B

We are going to use bacteria from the pond mud to create and study a mini ecosystem called a Winogradsky column.  It is based on the idea of ecological balance what means cooperation between different types of bacteria. Residues produced by ones constitute substrate for others. First of all we mix the mud with several ingredients to provide food and other substrates for the bacteria to use. These ingredients are:

  • ·         30 g of mud.
  • ·         3’36 g of salt – to develop halophilic bacteria.
  • ·         Shredded paper – to provide Carbon source on the form of cellulose.
  • ·         0’99g of gypsum (Calcium sulphate – to provide a source of sulphur - and Calcium carbonate – as another source of carbon).
  • ·         0’17 g of Sodium bicarbonate - it will provide Carbon too.
  • ·         Pond water

Then, we add the mix to the test tube (1/3 of the tube), we add a mix of sediment and mud (2/3) and we seal it. We place the column next to a window for several weeks.

We expect it to develop halophilic bacteria, some anaerobic bacteria and some aerobic bacteria. Some of them should be able to break down the cellulose in order to give glucose.

Finally, we will post more blog entries to analyse what happens in our Winogradsky column week after week.

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