Pond mud→ 20,81g
Yeast extract→ 0,1g
Sodium sulfate→ 0,4g
Calcium sulfate → 0,1g
Pieces of paper→ 0,1g
For our Winogradsky column we use pond mud as a culture medium. The yeast extract can be used for microorganisms as a nutrient source because it is high in B vitamin, and it contains amino acids and other factors that are ideal for their growth.In addition, we have used sodium sulfate and calcium sulfate as a source of sulfur that will be used for the anaerobic microorganisms that grow at the bottom of our column in order to being able to accomplish their breathing process.Finally,we have added little pieces of paper that can be used as a carbon source (cellulose) so the organisms can obtain energy from the synthesis of glucose which is required for their biological processes.
As a result of all this, we predict that there will be able to notice a few layers.We will be able to find aerobic microorganisms as there is enough oxygen for them to use. Facultative anaerobic organisms will be found in the layers that are in between, and anaerobic organisms will be found in the deepest layer of our column.
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