Winogradsky column lab page!

Welcome to the Winogradsky column lab page! Students from the Departments of Biological Applications and Technology, University of Ioannina and Icthyology and Aquatic Environment, University of Thessaly, Greece and the Microbiology course, Faculty of Sciences, University of Cádiz, Spain, discuss their findings on Winogradsky columns they constructed!

If you want to add a post, please feel free to contact the blog administrators (Hera Karayanni, Sokratis Papaspyrou or Kostas Kormas)!

Καλωσορίσατε στη σελίδα των Winobloggers! Διαδικτυακός τόπος συνάντησης φοιτητών, φοιτητριών και διδασκόντων δύο Τμημάτων από την Ελλάδα: Tμήμα Βιολογικών Εφαρμογών και Τεχνολογιών, Παν/μιο Ιωαννίνων και Τμήμα Γεωπονίας, Ιχθυολογίας και Υδάτινου Περιβάλλοντος, Παν/μιο Θεσσαλίας και ενός από την Ισπανία: Σχολή Θετικών Επιστημών, Πανεπιστήμιο του Cadiz. Παρακολουθούμε, σχολιάζουμε, ρωτάμε, απαντάμε σχετικά με τα πειράματά μας, τις στήλες Winogradsky!

Bienvenidos a la pagina web de los Winobloggers! Aquí los estudiantes y profesores de dos departamentos griegos, el Departamento de Aplicaciones y Tecnologías Biológicas de la Universidad de Ioannina y el Departmento de Agricultura, Ictiología y Sistemas Acuáticos de la Universidad de Thessalia, junto con los estudiantes de Microbiología de la Facultad de Ciencias en la Universidad de Cádiz, se reúnen para observar, comentar, preguntar y responder a preguntas relacionadas con nuestro experimento, la columna Winogradsky.

Winogradksy columns

Winogradksy columns
'In the field of observation, chance only favors the prepared mind' Pasteur 1854

Blog posts

Sunday, 21 May 2017

UCA_1C_Week 6

                                                         SECOND POST

Hello! It´s been six weeks since we prepared our column and now it´s time to talk about the changes we have seen. Here we have some photos.

As we can see, at the bottom of the column it has appeared a black colour joined with a pink colour. We suppose that in this area there is not oxygen, so we can find microorganisms which are able to make fermentative processes.

The waste of these microorganims is used by bacteria that reduce the sulphate. The result of this process is the liberation of sulphides. It goes to the top of the column which is oxygenated by cyanobacteria and algae.

Also, there are photosynthetic bacteria that use the sulphur we can find in the column due to the sulphide gradient.

                                                                                          Victoria, Andrea, Alessia.


  1. Hi,
    I see a smal mixup of ideas... :-)
    1. what is the casue of the pink colour in the middle of the very black sediment you have atthe bootom?
    2. What is producing the black colour?
    3. Sulfides are not oxidised by cyanobacteria or algae (Sulfide is toxic to them). What hapens to the sulfide difusing upwards as you correctly say?
    4. I see a lot of light brown colour in your column. What is that?

    Questions quetions questions...

    1. 1. We suppose that the pink colour is because there are purple bacteria and bacteria that reduced de CaSO4.

      2. At the bottom of the column there is not oxygen, so we can find microorganisms which are able to make fermentative processes using sulfhate. In this process, it is produced SH2. A part of SH2 has reacted with the iron that we can find in the sediment we added and the result has been SFe (black).

      3. Sulfide is used by bacteria which are in the middle of the column. They use SH2 as reducer, CO2 and chemical energy from light to produce their organic substance.
      4. The light Brown colour is the sediments we added.