Winogradsky column lab page!

Welcome to the Winogradsky column lab page! Students from the Departments of Biological Applications and Technology, University of Ioannina and Icthyology and Aquatic Environment, University of Thessaly, Greece and the Microbiology course, Faculty of Sciences, University of Cádiz, Spain, discuss their findings on Winogradsky columns they constructed!

If you want to add a post, please feel free to contact the blog administrators (Hera Karayanni, Sokratis Papaspyrou or Kostas Kormas)!

Καλωσορίσατε στη σελίδα των Winobloggers! Διαδικτυακός τόπος συνάντησης φοιτητών, φοιτητριών και διδασκόντων δύο Τμημάτων από την Ελλάδα: Tμήμα Βιολογικών Εφαρμογών και Τεχνολογιών, Παν/μιο Ιωαννίνων και Τμήμα Γεωπονίας, Ιχθυολογίας και Υδάτινου Περιβάλλοντος, Παν/μιο Θεσσαλίας και ενός από την Ισπανία: Σχολή Θετικών Επιστημών, Πανεπιστήμιο του Cadiz. Παρακολουθούμε, σχολιάζουμε, ρωτάμε, απαντάμε σχετικά με τα πειράματά μας, τις στήλες Winogradsky!

Bienvenidos a la pagina web de los Winobloggers! Aquí los estudiantes y profesores de dos departamentos griegos, el Departamento de Aplicaciones y Tecnologías Biológicas de la Universidad de Ioannina y el Departmento de Agricultura, Ictiología y Sistemas Acuáticos de la Universidad de Thessalia, junto con los estudiantes de Microbiología de la Facultad de Ciencias en la Universidad de Cádiz, se reúnen para observar, comentar, preguntar y responder a preguntas relacionadas con nuestro experimento, la columna Winogradsky.

Winogradksy columns

Winogradksy columns
'In the field of observation, chance only favors the prepared mind' Pasteur 1854

Blog posts

Wednesday, 7 June 2017

POST 1, group 2A UCA

First day, 

Hello, we are a group of young biotechnology students in UCA (Cádiz University) and we have done the project of Winowradsky Column.

The Winowradsky Column is an experiment which can create the necessary conditions for the nutrition and reproduction of many microorganism.
With this experiment we can study a microsystem and the different types of bacteria who live in as well ass its energy source.

In the column we add the next composition.

Nutrient data

We have take 20,85 grams of sediment of San Pedro River´s and have added:
  • Cellulose: 0,24 g.
  • NaCl: 0,30 g.
  • Na2SO4: 0,32 g.
  • Plaster: 0,16 g.
  • Agar: 0,55 g.
  • Calcium Carbonate: 0,14 g.
  • Calcium Sulfate: 0,44 g.
  • Glucose: 0,48 g.

When we made the mix, we added 0,63 grams of sand, 20,27 grams of mud and plot water. Finally we covert the column and we put it near windows.

We expect that in a few weeks different types of bacteria appear. According with mix should appear halo bacteria for NaCl, also there are a lot bacteria capable of metabolizing cellulose like Clostridium Thermocellum.

We have also added Agar that provides consistency and food to many bacteria and CaCO3 that would serve as carbon source for organism.

We supone that in the top there will be photosynthetic bacteria which use the sunlightt like energyy source and finally at the bottom there will be anaerobic bacteria which use NaSO4 and calcium sulfate like energyy source doing fermentativas process.

We hope you will continue the process with us. In a few weeks we will report to the evolution of column. See you latter! :).

1 comment:

  1. Hay hay.... although you highlight the main points, the post is written in a rush (many typos...).

    You added agar as a C source. Actually agar is being used in Petri dishes precisely because not many microorganisms can degrade it. So you definitely enriched for agar-degraders.