Winogradsky column lab page!

Welcome to the Winogradsky column lab page! Students from the Departments of Biological Applications and Technology, University of Ioannina and Icthyology and Aquatic Environment, University of Thessaly, Greece and the Microbiology course, Faculty of Sciences, University of Cádiz, Spain, discuss their findings on Winogradsky columns they constructed!

If you want to add a post, please feel free to contact the blog administrators (Hera Karayanni, Sokratis Papaspyrou or Kostas Kormas)!

Καλωσορίσατε στη σελίδα των Winobloggers! Διαδικτυακός τόπος συνάντησης φοιτητών, φοιτητριών και διδασκόντων δύο Τμημάτων από την Ελλάδα: Tμήμα Βιολογικών Εφαρμογών και Τεχνολογιών, Παν/μιο Ιωαννίνων και Τμήμα Γεωπονίας, Ιχθυολογίας και Υδάτινου Περιβάλλοντος, Παν/μιο Θεσσαλίας και ενός από την Ισπανία: Σχολή Θετικών Επιστημών, Πανεπιστήμιο του Cadiz. Παρακολουθούμε, σχολιάζουμε, ρωτάμε, απαντάμε σχετικά με τα πειράματά μας, τις στήλες Winogradsky!

Bienvenidos a la pagina web de los Winobloggers! Aquí los estudiantes y profesores de dos departamentos griegos, el Departamento de Aplicaciones y Tecnologías Biológicas de la Universidad de Ioannina y el Departmento de Agricultura, Ictiología y Sistemas Acuáticos de la Universidad de Thessalia, junto con los estudiantes de Microbiología de la Facultad de Ciencias en la Universidad de Cádiz, se reúnen para observar, comentar, preguntar y responder a preguntas relacionadas con nuestro experimento, la columna Winogradsky.

Winogradksy columns

Winogradksy columns
'In the field of observation, chance only favors the prepared mind' Pasteur 1854

Blog posts

Thursday, 1 June 2017

UCA_3C_1: DAY 1


Our column contains mud from Rio San Pedro’s river, yeast extract (0.1g), calcium sulfate (0.16g), marine water and sand.

We are Juan, Jesus and Carlos, students from Biotechnology in Cadiz, and we are going to introduce you our Winogradsky column. We’ll be commentating on the evolution of the column with the passage of time.

In order to elaborate the column, we followed the next process:

  1. We select he nutrients and weigh them.
  2. We take 20.25 g of mud.
  3. We mix nutrients and mud with marine water.
  4. We put the mixture in a glass tube.We take 59.52 g of sand and 25.02 g of mud apart and we put it in the tube mixed with marine water.
  5. We let it quiet and wait for a stratification for some weeks

We expect to obtain stratification in the organisms that live in the mud.

First of all, in the lowest layer we expect to obtain quimiosinthetic bacterias, which feed on sulfate ion present of the CaSO4. They will break down it into sulfite ion, which is used for the bacterias above.
The cycle goes on until the sulfur is completely oxidized, in the shape of sulfidric acid.
Finally, we think that the aerobic organisms will be inclined to move for the top of the tube where is the only place where there is oxygen.

Here you are how the column was the first day:

1 comment:

  1. Hi,
    "obtain quimiosinthetic bacterias, which feed on sulfate ion present of the CaSO4"
    Bacteria that use sulfate to respire are not chemoautotrophs, but chemohetereotrophs.

    "until the sulfur is completely oxidized, in the shape of sulfidric acid."
    Sulfate is reduced, not oxidised, to hydrogen sulfide, a more reduced form.

    I suppose the pictures are not from teh first day as I can see some different zones have developed. Which day was this picture taken?