Winogradsky column lab page!

Welcome to the Winogradsky column lab page! Students from the Departments of Biological Applications and Technology, University of Ioannina and Icthyology and Aquatic Environment, University of Thessaly, Greece and the Microbiology course, Faculty of Sciences, University of Cádiz, Spain, discuss their findings on Winogradsky columns they constructed!

If you want to add a post, please feel free to contact the blog administrators (Hera Karayanni, Sokratis Papaspyrou or Kostas Kormas)!

Καλωσορίσατε στη σελίδα των Winobloggers! Διαδικτυακός τόπος συνάντησης φοιτητών, φοιτητριών και διδασκόντων δύο Τμημάτων από την Ελλάδα: Tμήμα Βιολογικών Εφαρμογών και Τεχνολογιών, Παν/μιο Ιωαννίνων και Τμήμα Γεωπονίας, Ιχθυολογίας και Υδάτινου Περιβάλλοντος, Παν/μιο Θεσσαλίας και ενός από την Ισπανία: Σχολή Θετικών Επιστημών, Πανεπιστήμιο του Cadiz. Παρακολουθούμε, σχολιάζουμε, ρωτάμε, απαντάμε σχετικά με τα πειράματά μας, τις στήλες Winogradsky!

Bienvenidos a la pagina web de los Winobloggers! Aquí los estudiantes y profesores de dos departamentos griegos, el Departamento de Aplicaciones y Tecnologías Biológicas de la Universidad de Ioannina y el Departmento de Agricultura, Ictiología y Sistemas Acuáticos de la Universidad de Thessalia, junto con los estudiantes de Microbiología de la Facultad de Ciencias en la Universidad de Cádiz, se reúnen para observar, comentar, preguntar y responder a preguntas relacionadas con nuestro experimento, la columna Winogradsky.

Winogradksy columns

Winogradksy columns
'In the field of observation, chance only favors the prepared mind' Pasteur 1854

Blog posts

Wednesday, 7 June 2017


Hi! We are Jorge, Alejandro and Pepe and we're studying Biotechnology at UCA. We have been
working on a Winogradsky column as part of microbiology practicals so we are glad to show you
how it has evolved since we prepared it.
Firstable, we are going to explain what is our column made of and which are our suppositions about
its development based on the composition:
– The main sustances that compose the colum are sand and mud from Río San Pedro. Mud
contains lots of metals and minerals which are necessary in the development of the
– As carbon source we have added 0,4 g of cellulose. It will be used by chemoheterotrophs
microorganisms to grow but due to the difficulties related to its metabolism ( it's a polymer
that needs to be degraded first to its monomers ) it will take some time.
– We've also added 0,4 g of Na2SO4 that provides sulphate to sulphur-reducing bacteria during
the anaerobic respiration, generating H2S.
– Next we added 0,3 g of CaCO3. It will supply inorganic carbon to photosynthetic
microorganisms and will act as buffer solution keeping a constant pH.
– Finally we filled up the colum with sea water, we wrapped it up with cling film and left it in
the lab under the sunlight.
As we have prepared a column with a balanced composition we hope to observe a varied ecosystem
with lots of different types of microorganisms. On the top where there is plentiful of oxygen aerobic
photoautotrophs microorganisms as well as aerobic heterotrophs microorganisms will grow.
At the same time we descend in the column, oxygenn concentration decreases in contrast to H2S's which increases creating an O2- H2S gradient. Because of this fact, in the lower zone of the column
only anaerobic microorganisms will grow depending on their compatibility to sulphide levels.

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