Materials:
- 20 g of Rio San Pedro’s sediment.
- Destilled water/sea water (group 4) and sea water (group
3).
- 0,3 grammes of filter paper (both groups)
- 0,1 grammes of CaSO4 and 0,3 grammes of SO4- (group 3)
- 100 grammes of sand.
Objectives.
The objective of this experiment is to recognise the different
microorganisms depending on their metabolism, nutrition, etc. For that, we choose
the nutrients and environment of the column depending on the microorganisms that
we want to benefit.
Preparation:
We add 20 grammes of Rio san pedro’s sediment in a plastic
recipient. With the purpose of getting it viscous, we mix it with some water. Each
group add their chosen products (paper, sulfate…), and we keep mixing and adding
water until necessary. We achieve a thick mass that we can get into the tube
with the help of a funnel. Then we hit it softly so the sediment will go down
on the tube, and the trapped bubbles go away. We mark the tube to indicate the
level of the preparation, so we know where the nutrients are.
We prepare another mix with 20 grammes of sediment, 100
grammes of sand and some water, and we put it into the tube. On the top of the
column, we add some more water (2 cm).
Hypothesis
We think that , in the column with only filter paper, are
going to grow the chemoorganoheterotroph organisms, and, in the other hand, in
the column with sulfate, are going to grow the chemolithoheterotroph organisms.
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