Winogradsky column lab page!

Welcome to the Winogradsky column lab page! Students from the Departments of Biological Applications and Technology, University of Ioannina and Icthyology and Aquatic Environment, University of Thessaly, Greece and the Microbiology course, Faculty of Sciences, University of Cádiz, Spain, discuss their findings on Winogradsky columns they constructed!

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Καλωσορίσατε στη σελίδα των Winobloggers! Διαδικτυακός τόπος συνάντησης φοιτητών, φοιτητριών και διδασκόντων δύο Τμημάτων από την Ελλάδα: Tμήμα Βιολογικών Εφαρμογών και Τεχνολογιών, Παν/μιο Ιωαννίνων και Τμήμα Γεωπονίας, Ιχθυολογίας και Υδάτινου Περιβάλλοντος, Παν/μιο Θεσσαλίας και ενός από την Ισπανία: Σχολή Θετικών Επιστημών, Πανεπιστήμιο του Cadiz. Παρακολουθούμε, σχολιάζουμε, ρωτάμε, απαντάμε σχετικά με τα πειράματά μας, τις στήλες Winogradsky!

Bienvenidos a la pagina web de los Winobloggers! Aquí los estudiantes y profesores de dos departamentos griegos, el Departamento de Aplicaciones y Tecnologías Biológicas de la Universidad de Ioannina y el Departmento de Agricultura, Ictiología y Sistemas Acuáticos de la Universidad de Thessalia, junto con los estudiantes de Microbiología de la Facultad de Ciencias en la Universidad de Cádiz, se reúnen para observar, comentar, preguntar y responder a preguntas relacionadas con nuestro experimento, la columna Winogradsky.

Winogradksy columns

Winogradksy columns
'In the field of observation, chance only favors the prepared mind' Pasteur 1854

Blog posts

Monday, 19 March 2018

UCA_3C, 4C_1: Day 1. Column’s preparation.

- 20 g of Rio San Pedro’s sediment.
- Destilled water/sea water (group 4) and sea water (group 3).
- 0,3 grammes of filter paper (both groups)
- 0,1 grammes of CaSO4 and 0,3 grammes of SO4- (group 3)
- 100 grammes of sand.
The objective of this experiment is to recognise the different microorganisms depending on their metabolism, nutrition, etc. For that, we choose the nutrients and environment of the column depending on the microorganisms that we want to benefit.
We add 20 grammes of Rio san pedro’s sediment in a plastic recipient. With the purpose of getting it viscous, we mix it with some water. Each group add their chosen products (paper, sulfate…), and we keep mixing and adding water until necessary. We achieve a thick mass that we can get into the tube with the help of a funnel. Then we hit it softly so the sediment will go down on the tube, and the trapped bubbles go away. We mark the tube to indicate the level of the preparation, so we know where the nutrients are.
We prepare another mix with 20 grammes of sediment, 100 grammes of sand and some water, and we put it into the tube. On the top of the column, we add some more water (2 cm).
We think that , in the column with only filter paper, are going to grow the chemoorganoheterotroph organisms, and, in the other hand, in the column with sulfate, are going to grow the chemolithoheterotroph organisms.

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