Winogradsky column lab page!

Welcome to the Winogradsky column lab page! Students from the Departments of Biological Applications and Technology, University of Ioannina and Icthyology and Aquatic Environment, University of Thessaly, Greece and the Microbiology course, Faculty of Sciences, University of Cádiz, Spain, discuss their findings on Winogradsky columns they constructed!

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Καλωσορίσατε στη σελίδα των Winobloggers! Διαδικτυακός τόπος συνάντησης φοιτητών, φοιτητριών και διδασκόντων δύο Τμημάτων από την Ελλάδα: Tμήμα Βιολογικών Εφαρμογών και Τεχνολογιών, Παν/μιο Ιωαννίνων και Τμήμα Γεωπονίας, Ιχθυολογίας και Υδάτινου Περιβάλλοντος, Παν/μιο Θεσσαλίας και ενός από την Ισπανία: Σχολή Θετικών Επιστημών, Πανεπιστήμιο του Cadiz. Παρακολουθούμε, σχολιάζουμε, ρωτάμε, απαντάμε σχετικά με τα πειράματά μας, τις στήλες Winogradsky!

Bienvenidos a la pagina web de los Winobloggers! Aquí los estudiantes y profesores de dos departamentos griegos, el Departamento de Aplicaciones y Tecnologías Biológicas de la Universidad de Ioannina y el Departmento de Agricultura, Ictiología y Sistemas Acuáticos de la Universidad de Thessalia, junto con los estudiantes de Microbiología de la Facultad de Ciencias en la Universidad de Cádiz, se reúnen para observar, comentar, preguntar y responder a preguntas relacionadas con nuestro experimento, la columna Winogradsky.

Winogradksy columns

Winogradksy columns
'In the field of observation, chance only favors the prepared mind' Pasteur 1854

Blog posts

Friday, 9 March 2018

UCA_5A-6A-7A_1: Day 0 – Starting Winogradsky Column’s project!

Winogradsky Column Materials:

All groups included 40g Rio San Pedro sediment, 100g sand and sea water.

5A group’s column: 0,31g tryptone; 0,62g powdered milk; 0,1g CaSO4.

6A group’s column: 0,74g agar, 0,74g CaCO3.

7A group’s column: 0,1g CaSO4, 0,3g paper (cellulose).


1º-. We took off the solids residues from the sediment. Then, we mixed 20g of Rio San Pedro sediment with sea water, and after that we included the previous materials mentioned for each group.

2º-. Once the mixture was homogenized, we put it into a test tube. Secondly, we mixed 100g of sand and 20g Rio San Pedro sediment, with sea water. Both mixtures’ texture should be like milkshake’s texture. 

3º-. After that, we added the second mixture to test tube until we filled up to 2/3 of the test tube. We left the mixture stand a little bit. 

4º-. Finally, we added 2-3 cm of sea water on the top. 


5A: When the project has finished, we won't find aerobic bacterias owing to the plenty sources of N and S. In our column, two predominant colors will be found. In the lowest part of the test tube, there will be a black color which is caused by bacterias with a more anaerobic metabolism (respiration S2O3- -> H2S). It will take up about 1/3 of the solid part of the column. Above it, we will find a grey color, which is caused by other anaerobic bacterias (respiration NO3- -> NO2- -> N2).

6A: In conclusion, after having thrown to the column CaCO3 (calcium carbonate) and Agar the sample will keep the color it has because it does not have sulfur and although this in the dark it will not turn blue which would be the color it must acquire when the bacteria feed on sulfur.

7A: We thought that our column will have aerobic and anaerobic bacterias, due to the two feeding sources, the cellulose from the paper and the CaSO4. We also thought that our column will change its color because we will have anaerobic bacterias producing H2S from SO4-. Then the H2S will react with the iron in the mud to produce a dark-blue color. Besides, our column is exposed to sunlight. 

We will report more about our columns in the next entry coming soon.

 A5 Winogradksy Column.
 A6 Winogradsky Column
 A7 Winogradsky Column

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