Winogradsky column lab page!

Welcome to the Winogradsky column lab page! Students from the Departments of Biological Applications and Technology, University of Ioannina and Icthyology and Aquatic Environment, University of Thessaly, Greece and the Microbiology course, Faculty of Sciences, University of Cádiz, Spain, discuss their findings on Winogradsky columns they constructed!

If you want to add a post, please feel free to contact the blog administrators (Hera Karayanni, Sokratis Papaspyrou or Kostas Kormas)!

Καλωσορίσατε στη σελίδα των Winobloggers! Διαδικτυακός τόπος συνάντησης φοιτητών, φοιτητριών και διδασκόντων δύο Τμημάτων από την Ελλάδα: Tμήμα Βιολογικών Εφαρμογών και Τεχνολογιών, Παν/μιο Ιωαννίνων και Τμήμα Γεωπονίας, Ιχθυολογίας και Υδάτινου Περιβάλλοντος, Παν/μιο Θεσσαλίας και ενός από την Ισπανία: Σχολή Θετικών Επιστημών, Πανεπιστήμιο του Cadiz. Παρακολουθούμε, σχολιάζουμε, ρωτάμε, απαντάμε σχετικά με τα πειράματά μας, τις στήλες Winogradsky!

Bienvenidos a la pagina web de los Winobloggers! Aquí los estudiantes y profesores de dos departamentos griegos, el Departamento de Aplicaciones y Tecnologías Biológicas de la Universidad de Ioannina y el Departmento de Agricultura, Ictiología y Sistemas Acuáticos de la Universidad de Thessalia, junto con los estudiantes de Microbiología de la Facultad de Ciencias en la Universidad de Cádiz, se reúnen para observar, comentar, preguntar y responder a preguntas relacionadas con nuestro experimento, la columna Winogradsky.

Winogradksy columns

Winogradksy columns
'In the field of observation, chance only favors the prepared mind' Pasteur 1854

Blog posts

Tuesday, 6 March 2018

UCA_5C_1:26/02/2018_0,5g of dextrose+ Río San Pedro sediment. UCA_6C_1:26/02/2018_0,25g CaSO4+ 0,25g paper + Río San Pedro sediment. UCA_7C_1:26/02/2018_0,5g of NaCl+ Río San Pedro sediment.

Hi everyone!
We are all students of 1º year of Biotechnology. In this post, we are going to describe our Winogradsky column experiment.
In first place, we weighted 20 g of sediment and mixed it with salt water so that it had a liquid texture like a smoothie. After doing that, we tipped out the mixture into the test tube being careful with the air bubbles.
Secondly, we weighted and mixed 20 g of sediment with 100g of soil until they become an homogeneous mix. Later, we added the blend to the test tube avoiding the formation of air bubbles. We eliminated the bubbles formed bumping the test tube against the workplace so that they could come out of the column.
We also had to add 2-3 ml of water above the semisolid mash and we had to leave 2 cm empty at the top of the column so that we could close the test tube. We repeat this process three times. In each column, we added differents substances:
C5: 0,5g dextrose.
C6: 0,25g CaSO4+ 0,25g paper.
C5: 0,5g of NaCl.
The column was placed in a luminous area.
We have an hypothesis of what can occur a week after preparing the column. We think that:
In C5 (0,5g dextrose):
Although now the C5 ´s column Winogradsky   is the darkest (due to the fact that it presents in his composition organic matter), in the future it  will have a clearer color. We base on two reasons:
It will be exposed to the solar light, that’s why the  photosynthetic organisms will predominate in the column.
·         It is highly probable that the quantity of initial glucose is close to ending. This means that  the heterotrophs organisms aren´t going to have food source. In the other hand, autotrophs organisms generate more organic matter.

In C6 (0,25g CaSO4+ 0,25g paper):
·         At the superior surface of the column there will be aerobial phototrophs, because hera there will be more oxigen than on the bottom.
·         Around the rest of the column there will be anaerobial phototrophs.
·         In the interior of the column there will be heterotrophs.
·         In the inferior region of our column there will be organotrophs.
·         At the top, there will be lithotrophs.
In C7 (0,5g of NaCl):
Our hypothesis is that the most of microorganisms are autotrophs because we have improved the conditions for growing autotrophy’s microorganisms. We think that in the surface of the test tube will grow photoautotrophs’ microorganisms because our Winogradksy column is near to the sun light. At the bottom,  almost all the microorganisms will be anaerobics because there will not be oxygen enough for aerobic breath.

Ilustración 1:C5
 Ilustración 2: C6

Ilustración 3:C7

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