Hi everyone!
We are all students of 1º year of Biotechnology. In this
post, we are going to describe our Winogradsky column experiment.
In first place, we weighted 20 g of sediment and mixed it
with salt water so that it had a liquid texture like a smoothie. After doing
that, we tipped out the mixture into the test tube being careful with the air
bubbles.
Secondly, we weighted and mixed 20 g of sediment with 100g
of soil until they become an homogeneous mix. Later, we added the blend to the
test tube avoiding the formation of air bubbles. We eliminated the bubbles
formed bumping the test tube against the workplace so that they could come out
of the column.
We also had to add 2-3 ml of water above the semisolid mash
and we had to leave 2 cm empty at the top of the column so that we could close
the test tube. We repeat this process three times. In each column, we added
differents substances:
C5: 0,5g dextrose.
C6: 0,25g CaSO4+ 0,25g paper.
C5: 0,5g of NaCl.
The column was placed in a luminous area.
We have an hypothesis of what can occur a week after preparing
the column. We think that:
In C5 (0,5g dextrose):
Although now the C5 ´s column Winogradsky is the darkest (due to the fact that it
presents in his composition organic matter), in the future it will have a clearer color. We base on two
reasons:
It will be exposed to the solar light,
that’s why the photosynthetic organisms
will predominate in the column.
·
It is highly probable that the quantity of
initial glucose is close to ending. This means that the heterotrophs organisms aren´t going to
have food source. In the other hand, autotrophs organisms generate more organic
matter.
In C6 (0,25g CaSO4+ 0,25g paper):
·
At the superior surface of the column there will
be aerobial phototrophs, because
hera there will be more oxigen than on the bottom.
·
Around the rest of the column there will be anaerobial phototrophs.
·
In the interior of the column there will be heterotrophs.
·
In the inferior region of our column there will
be organotrophs.
·
At the top, there will be lithotrophs.
In C7 (0,5g of NaCl):
Our hypothesis is that the most of microorganisms are autotrophs
because we have improved the conditions for growing autotrophy’s microorganisms.
We think that in the surface of the test tube will grow photoautotrophs’
microorganisms because our Winogradksy column is near to the sun light. At the
bottom, almost all the microorganisms
will be anaerobics because there will not be oxygen enough for aerobic breath.
Ilustración 1:C5
Ilustración 3:C7
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