Helo! We are Rafael Pardo Velasco, Jaime Pérez Leiva, Blanca Ruiz Alonso, María Oliva Pareja González, Covadonga Muñoz Raya and Lorena Rodríguez Rivero! We will explain how we have made our Winogradsky column!
We have made our
Winogradsky colum with:
Rio San Pedro
sediment, 0,5g of CaCO3 and filter paper.
That colum has been
made for this way:
- We put 20g of
sediment and much water in a plastic container. When all the
compounds became viscous, we put it in a test tube, covering ⅓ of
it.
- We put slowly 0,5g
of CaCO3 and much filter paper inside the tube. Before we
had mixed them without introducing air bubbles.
- We carefuly hit
the tube to send the sediment to the botton of the tube and to catch
all the air bubbles.
-We washed the
plastic container.
- We mixed 100g of
sand and 20g of sediment without enriching.
- We repeated the
third step and cleaned the tube borders.
- We waited for
until all the compounds proned.
- A water layer was
formed on the top of the tube.
Observations:
Because of the addition of the filter paper and the CaCO3, we have created an environment that is enriched in CO2, furthermore, mixing all the compounds of our column, have ensured a fast diffusion of nutrients and gases inside the tube, so gradients are more widespread.
We made two Winogradsky columns for the same way to study the influence of light on them, putting one of our columns in light and the other in darkness. We supose that, in the column that we have put in darkness, it will inhibit the growth of photoautotrophic microorganisms. So, that column will only depend on quimioautotrophic microoorganisms, that pick up its energy of carbonated and sulfurated compounds. The other column will depend on light too.
What about heterotrophic bacteria? Where will you find them?
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