University of Thessaly
Group number: 20 (Papadopoulou Aikaterini-Papaioannou Evangelia-Anna)
Day number: 45
Incubation: Dark

 Winogradsky colum

Hello to everyone

·                     DAY 14 (4-4-16)

                                        Fig 1  

                                                      Fig 2

·                     DAY 17 (7-4-16):

                              Fig 3

·                     DAY 21 (11-4-16):

 
  Fig 4  

                                                                                                         
Fig 5

·                     DAY 24 (14-4-16):

Fig 6   

                                                                                                
  Fig 7

·                     DAY 28 (18-4-16):

                                

                                   fig 8                                                                  

fig 9

·                     DAY 31 (21-4-16):

fig10

fig 11

·                     DAY 35(25-4-16)

fig 12

fig 13                                                  

    On the 14th day of the experiment (Fig.1)we observed a change in the liquid phas eof the experiment in an intense beige color with white and black colors probably due to the start of the degradative activity of microorganisms and the presence of suspended particles. The precipitate has been tarnished almost entirely due to the iron and other reasons which we have mentioned in our previous post. While the odor has become unpleasant  because of hydrogen sulfide in the column. Also on the surface (Fig 2) growth of the foam was observed and existence of bubbles which may suggest the presence of intense microorganism action and oxygen trapped.

  

  On the 17th (Fig 3) of the experiment change a change was observed in the color of the liquid phase. A beige-brown color replaced the previous beige one, whilst the white foam along with the bubbles remain in the surface. Also the precipitate showed no significant change compared to the previous count while the upper column was a white layer probably due to calcium, which is present in large quantities in seawater.

  On day 21 of the experiment the only change observed  was in the water column surface (Figure 5) where the foam has taken the form of crust. This is probably a result  of the calcium carbonate which is contained in one of the enrichment materials used in this experiment (egg).
  

  On day 24 of the experiment  the precipitate seems to start losing its beige coloration in the water column (Fig 6) and the foam-bubble crust on the surface (Fig 7). This might be a result of  the death of the microorganisms present  resulting in the lack of oxygen.

On day 28 of the experiment seems  the beige color in the water column (Fig 8) becomes  even lighter for the aforementioned reasons . The same applies to the surface of the water column. It was also observed that the upper column of the white precipitate layer disappeared.
   

  On the 31st day of the experiment the reduction of  the foam-bubble crust (Fig.11) is more noticeable than the changing of the color of the water column, which does not appear to have any change compared to the previous measurement (Fig.10)

The 35th day of the experiment observed reappearance of foam-bubble crust (Fig.13) with small changes in the color of the water column (Fig.12)

Notably, the odor remains strong in all the above measurements.