First day,
Hello, we are a group of young biotechnology students in UCA (Cádiz University) and we have done the project of Winowradsky Column.
The Winowradsky Column is an experiment which can create the necessary conditions for the nutrition and reproduction of many microorganism.
With this experiment we can study a microsystem and the different types of bacteria who live in as well ass its energy source.
In the column we add the next composition.
Nutrient data
We have take 20,85 grams of sediment of San Pedro River´s and have added:
- Cellulose: 0,24 g.
- NaCl: 0,30 g.
- Na2SO4: 0,32 g.
- Plaster: 0,16 g.
- Agar: 0,55 g.
- Calcium Carbonate: 0,14 g.
- Calcium Sulfate: 0,44 g.
- Glucose: 0,48 g.
When we made the mix, we added 0,63 grams of sand, 20,27 grams of mud and plot water. Finally we covert the column and we put it near windows.
We expect that in a few weeks different types of bacteria appear. According with mix should appear halo bacteria for NaCl, also there are a lot bacteria capable of metabolizing cellulose like Clostridium Thermocellum.
We have also added Agar that provides consistency and food to many bacteria and CaCO3 that would serve as carbon source for organism.
We supone that in the top there will be photosynthetic bacteria which use the sunlightt like energyy source and finally at the bottom there will be anaerobic bacteria which use NaSO4 and calcium sulfate like energyy source doing fermentativas process.
We hope you will continue the process with us. In a few weeks we will report to the evolution of column. See you latter! :).
Hay hay.... although you highlight the main points, the post is written in a rush (many typos...).
ReplyDeleteYou added agar as a C source. Actually agar is being used in Petri dishes precisely because not many microorganisms can degrade it. So you definitely enriched for agar-degraders.