PREPARATION OF WINOGRADSKY COLUMN
16/03/2017
The Winograsky column constitutes
a model of aquatic ecosystem where we can see the bacterial photosynthesis and
the cycles of the sulfur produced by the metabolisms of the microorganisms that
proliferate in it.
In our case, we made two columns
from 60 grams of mud, 220 grams of sand and sea water. To prepare columns, we
began by preparing an enrichment mixture with nutrients favorable for the
development of microorganisms. This medium consisted of 1 gram of gypsum
(calcium sulphate), since it is a source of calcium and sulfur, and 0.54 grams
of dipotassium phosphate as it provides potassium and phosphorus.
Once this mixture was prepared,
we separated in equal parts to created two columns which would be subject to
different environmental conditions: one will be under direct sunlight
conditions and the other one in darkness. After a few weeks we will return to
visualize and comparte columns in order to observe if there are any differences
between them.
In the next image we could see
two columns, the one on the left was placed in the dark, and the one on the
right was put in sunlight.
So what is your hypothesis? Why did you use the specific compounds?
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