Winogradsky column lab page!

Welcome to the Winogradsky column lab page! Students from the Departments of Biological Applications and Technology, University of Ioannina and Icthyology and Aquatic Environment, University of Thessaly, Greece and the Microbiology course, Faculty of Sciences, University of Cádiz, Spain, discuss their findings on Winogradsky columns they constructed!

If you want to add a post, please feel free to contact the blog administrators (Hera Karayanni, Sokratis Papaspyrou or Kostas Kormas)!

Καλωσορίσατε στη σελίδα των Winobloggers! Διαδικτυακός τόπος συνάντησης φοιτητών, φοιτητριών και διδασκόντων δύο Τμημάτων από την Ελλάδα: Tμήμα Βιολογικών Εφαρμογών και Τεχνολογιών, Παν/μιο Ιωαννίνων και Τμήμα Γεωπονίας, Ιχθυολογίας και Υδάτινου Περιβάλλοντος, Παν/μιο Θεσσαλίας και ενός από την Ισπανία: Σχολή Θετικών Επιστημών, Πανεπιστήμιο του Cadiz. Παρακολουθούμε, σχολιάζουμε, ρωτάμε, απαντάμε σχετικά με τα πειράματά μας, τις στήλες Winogradsky!

Bienvenidos a la pagina web de los Winobloggers! Aquí los estudiantes y profesores de dos departamentos griegos, el Departamento de Aplicaciones y Tecnologías Biológicas de la Universidad de Ioannina y el Departmento de Agricultura, Ictiología y Sistemas Acuáticos de la Universidad de Thessalia, junto con los estudiantes de Microbiología de la Facultad de Ciencias en la Universidad de Cádiz, se reúnen para observar, comentar, preguntar y responder a preguntas relacionadas con nuestro experimento, la columna Winogradsky.

Winogradksy columns

Winogradksy columns
'In the field of observation, chance only favors the prepared mind' Pasteur 1854

Blog posts

Wednesday, 6 April 2016

UCA_5A_1: Day 28

Rio San Pedro sediment + 0.5g FeSO4 + filter paper

Few weeks later, it is noted a remarkable development and great evolution of the Winogradsky column.

First of all, at the dark side of the bottle, the bottom had been dispersed upwards; the orange lines we saw two weeks ago, now extended, occupying the entire area of the bottle.

Furthermore, there was a clear difference between the part of the column that received light and the one that did not.

The area did not received light was coloured black; we came to this conclusion with the help and the analysis of the bottle in complete dark conditions.
Besides this, at the top of the column could be seen filaments that were born from the sediment, spreading in the water.

Analysing the column and researching on the Internet, we could say that the light side is rich in O2 as we climb because of aerobic bacteria who usually develop in little lakes. They could be algae or photosynthetic cyanobacteria.

The column become anaerobic as far as we descend, being completely anaerobic at the bottom. In this way, we can find sulphate-oxidizers (Beggiatoa or Thiobacillus), purple non-sulphur bacteria (Rhodospirillum and Rhodopseudomonas) who produce these orange lines, purple sulphur bacteria (Chromatium), green sulphate bacteria (Chlorobium) with a green colour, sulphate-reducers (Desulfovibrio) who use by-products other bacteria to produce large amounts of SH2 and finally, we can find cellulose fermenters.

We could conclude that the amount of sulphur descended downwards at the dark side.

1 comment:

  1. Sounds great. Did you observe all these types of bacteria in your columns? I can only see black spots and rusty patches.