Winogradsky column lab page!


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Welcome to the Winogradsky column lab page! Students from the Departments of Biological Applications and Technology, University of Ioannina and Icthyology and Aquatic Environment, University of Thessaly, Greece and the Microbiology course, Faculty of Sciences, University of Cádiz, Spain, discuss their findings on Winogradsky columns they constructed!

If you want to add a post, please feel free to contact the blog administrators (Hera Karayanni, Sokratis Papaspyrou or Kostas Kormas)!



Καλωσορίσατε στη σελίδα των Winobloggers! Διαδικτυακός τόπος συνάντησης φοιτητών, φοιτητριών και διδασκόντων δύο Τμημάτων από την Ελλάδα: Tμήμα Βιολογικών Εφαρμογών και Τεχνολογιών, Παν/μιο Ιωαννίνων και Τμήμα Γεωπονίας, Ιχθυολογίας και Υδάτινου Περιβάλλοντος, Παν/μιο Θεσσαλίας και ενός από την Ισπανία: Σχολή Θετικών Επιστημών, Πανεπιστήμιο του Cadiz. Παρακολουθούμε, σχολιάζουμε, ρωτάμε, απαντάμε σχετικά με τα πειράματά μας, τις στήλες Winogradsky!


Bienvenidos a la pagina web de los Winobloggers! Aquí los estudiantes y profesores de dos departamentos griegos, el Departamento de Aplicaciones y Tecnologías Biológicas de la Universidad de Ioannina y el Departmento de Agricultura, Ictiología y Sistemas Acuáticos de la Universidad de Thessalia, junto con los estudiantes de Microbiología de la Facultad de Ciencias en la Universidad de Cádiz, se reúnen para observar, comentar, preguntar y responder a preguntas relacionadas con nuestro experimento, la columna Winogradsky.


Winogradksy columns

Winogradksy columns
'In the field of observation, chance only favors the prepared mind' Pasteur 1854

Blog posts

Saturday, 2 April 2016

Volos_Group 20_Day 12_Dark

University of Thessaly
Group number: 20 (Papadopoulou Aikaterini-Papaioannou Evangelia-Anna)
Day number: 12
Incubation: Dark




WinogradskyColumn


   We are a team of two people from the University of Thessaly in Greece and our experiment is Winogradsky column. The experimental procedure  involves:  the collection of sediment from the beach Anavros  (located  outside the city of  Volos) which  we subsequently  mixed with various enrichment materials. These materials were a hen's egg with a shell, which was used as a source of calcium and sulfur. In addition, pieces of newspaper were used  as a carbon source and cellulos. Then we took a plastic bottle (1,5 L) and we filled up to 2/3 with the sediment and the enrichment materials and then we supplemented with sea water (500 ml). Subsequently, the column created covered with clear film and placed in a dark place with relatively stable conditions.



Day 1  (21/3/2016)
Figure 1



Day 3  (24/3/2016)



Figure 2


Day 7  (28/3/2016)




                                                                                          
 Figure 3

Figure 4



Day 10  (31/3/2016)
                                                                  Figure 5




   On the first day of the experiment on 21/03/16 (Fig.1) held in Anavros Beach (Volos), the sample was taken, where the liquid phase separation (sea water was observed) from the solid occurred (sea sediment). The liquid phase was observed an intense yellow color derived from the enriched material, particularly egg, but no noticeable odor.
   On the third day (Fig. 2) we observed a slight decline in the bright yellow color in the liquid phase, and on the seventh day (Fig. 3) of the experiment we observed  a change in the color of the liquid phase to a yellowish white color in some points of the wall of the column in conjunction with a white  foam in the surface(Fig. 4) which may have been the outcome of oxygen trapped. Additionally, the sediment showed a relatively darker color change from the first day to the upper column tarnished, while the odor remained low.
   On the tenth day (Fig. 5) the lower portion of the column of the liquid phase obtained a white color while the surface was still a white foam. We also observed that the sediment had almost completely tarnished across the column and the unpleasant odor continued to be low. These events may indicate that the use of nutrients that have been chosen to put in Winogradsky column had an immediate effect on the growth of microorganisms and particulate matter. The black color of the upper pellet column can be attributed to the presence of organic material (egg) and the accumulation of by-products of metabolism. Finally, the odor may indicate the existence of hydrogen sulfide in the column.
  
 A new announcement will be made in the case of new changes

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