Winogradsky column lab page!

Welcome to the Winogradsky column lab page! Students from the Departments of Biological Applications and Technology, University of Ioannina and Icthyology and Aquatic Environment, University of Thessaly, Greece and the Microbiology course, Faculty of Sciences, University of Cádiz, Spain, discuss their findings on Winogradsky columns they constructed!

If you want to add a post, please feel free to contact the blog administrators (Hera Karayanni, Sokratis Papaspyrou or Kostas Kormas)!

Καλωσορίσατε στη σελίδα των Winobloggers! Διαδικτυακός τόπος συνάντησης φοιτητών, φοιτητριών και διδασκόντων δύο Τμημάτων από την Ελλάδα: Tμήμα Βιολογικών Εφαρμογών και Τεχνολογιών, Παν/μιο Ιωαννίνων και Τμήμα Γεωπονίας, Ιχθυολογίας και Υδάτινου Περιβάλλοντος, Παν/μιο Θεσσαλίας και ενός από την Ισπανία: Σχολή Θετικών Επιστημών, Πανεπιστήμιο του Cadiz. Παρακολουθούμε, σχολιάζουμε, ρωτάμε, απαντάμε σχετικά με τα πειράματά μας, τις στήλες Winogradsky!

Bienvenidos a la pagina web de los Winobloggers! Aquí los estudiantes y profesores de dos departamentos griegos, el Departamento de Aplicaciones y Tecnologías Biológicas de la Universidad de Ioannina y el Departmento de Agricultura, Ictiología y Sistemas Acuáticos de la Universidad de Thessalia, junto con los estudiantes de Microbiología de la Facultad de Ciencias en la Universidad de Cádiz, se reúnen para observar, comentar, preguntar y responder a preguntas relacionadas con nuestro experimento, la columna Winogradsky.

Winogradksy columns

Winogradksy columns
'In the field of observation, chance only favors the prepared mind' Pasteur 1854

Blog posts

Saturday, 2 April 2016

University of Thessaly, Department of Ichthyology and Aquatic Environment, Team 21, Papakonstantinou Angeliki & Paschou Maria, Day 12, Incubation: Light

In order to create our Winogradsky column we followed the procedure below:

1)  Sand sediment  was collected from the Anauros beach.

2) Mixed the sediment with enrichment materials (1 egg and pieces of newspaper).
3) The sediment was added in a plastic bottle and filled the 2/3 of the column.
4) After that we added sea water to the rest of the column.
5) The column was covered with transparent film.

We put the column near a window (which is bright enough) in relatively stable conditions. At the 1st day (21/3/16- pic.1) we can see that there is a clear separation between the liquid phase (sea water) and the solid phase (sea sediment). The liquid phase was observed and had a considerable turbidity with a yellowish color.


After 3 days (24/3/16-pic.2&3)the only thing that changed was the color of the upper layer of the sea water, which became slightly whiter.


At the 8th day of the experiment (28/3/16- pic. 4&5), on the upper layer of the column we observed a yellow foam with a relative increase in the turbidity of the liquid phase and a characteristic odor. The sediment showed a relatively darker color change from the first day. These facts may indicate the use of nutrients from the materials that have been chosen for the Winogradsky column, which leads to the growth of microorganisms and particulate matter. The light yellow color may be due to decomposition of organic matter (egg) and the accumulation of by-products of metabolism. Finally, the odor may be an indication of existence of hydrogen sulfide in the column.

                      pic.4                                           pic.5

Today (12th day-2/4/16) the surface of the column became whiter (pic.6) and the liquid phase has lost its yellow color (it has a rather grey color) and more particulate matter has settled above the sediment, (pic.7).

                       pic.6                                              pic.7              

We will inform you with further observations as soon as we see any noticeable changes in the column.

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